Effects of fermented wheat bran polysaccharides on lipid metabolism and antioxidant-related indexes of cholesterol-induced high fat zebrafish
LIU Cong-fu
WANG Rui-fang
QI Jing-wei
AN Xiao-ping
WANG Yuan
YANG Yan-ping
SONG Min
Abstract：The study was to investigate the effects of fermented wheat bran polysaccharides(FWBP)on the fat metabolism and antioxidant-related indicators in cholesterol-induced high-fat zebrafish.This study mainly used 5 dpf wild-type AB zebrafish as experimental animals.Firstly,a high-fat zebrafish model was constructed.It was clear that 5％high cholesterol diet could cause a significant increase in fat accumulation in zebrafish.On this basis,FWBP(0,0.25,0.50,0.75,1.00％o)was added to study whether it could improve cholesterol-induced fat metabolism and antioxidant dysfunction.Each group was subdivided into five replicates,with 30 zebrafishes per replicate.The experiment lasted for 10 days.The results showed that compared to the control group,the TC,TG,and LDL contentrations,as well as the activities of AST,ALT,and GGT of zebrafish in f 0.50‰ FWBP group were all significantly reduced(P＜0.05),the HDL content of fish in 1.00‰ FWBP group was significantly reduced(P＜0.05).Compared to the control group,the activities of SOD,CAT,and GSH-Px of fish in 0.25‰,0.50‰,and 0.75‰ FWBP groups were all significantly increased(P＜0.05),and the MDA content in all experimental groups was significantly decreased(P＜0.05).The study indicates that FWBP can effectively reduce the total fat and lipoprotein content in zebrafish after consuming high-fat feed,decrease the activity of enzymes related to fat metabolism and the content of TBIL,enhance the activity of antioxidant enzymes,reduce MDA content,alleviate oxidative stress caused by high fat,with the best effect observed at the addition of 0.50‰.
Keywords：fermented wheat bran polysaccharidehigh fat modelzebrafishfat metabolismantioxidation
Publication Date：2024-10-31
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 51-55 )