Optimization extraction and antioxidant activity of total saponins from Hericium erinaceus-Astragalus membranaceus residue mycoplasm
SONG Zhao-ran
SHI Xin
WU Yu-long
JIANG Hai-tao
LI Sheng-jie
HUO Guang-ming
HUA Chun
Abstract：This study aimed to optimize the extraction process of total saponins from the fungal matrix resulting from the dual solid-state fermentation of Hericium erinaceus-Astragalus membranaceus residue(HAMTS)and to determine its antioxidant activity.The content of total saponins before and after fermentation was measured using the vanillin method.A response surface method combined with ultrasonication was employed to optimize the extraction of HAMTS,and the reducing power as well as the scavenging ability against DPPH and ABTS+free radical of HAMTS were analyzed.The results showed that the content of HAMTS after fermentation was 10.34 mg/g,with an increase of 63.35％.Under the optimal extraction conditions:Liquid-to-solid ratio of 19 mL/g,ultrasonication time of 16 min,ethanol volume fraction of 74％,ultrasonic power of 62 W,the extraction amount of HAMTS was 11.48 mg/g.At a concentration of 10 g/L,the reducing power of HAMTS,as well as the DPPH and ABTS+free radical scavenging rates,were 0.80,96.48％,and 56.12％,respectively.The study indicates that the regression model established in the experiment can be used to predict the extraction amount of HAMTS,and HAMTS exhibits good antioxidant activity,demonstrating the potential for development as a feed additive.
Keywords：Hericium erinaceus-Astragalus membranaceus residue mycoplasmtotal saponinsoptimized extractionantioxidant activity
Publication Date：2024-09-15
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：7( 102-108 )