Optimization of extraction and clarification technology of Baitouweng oral liquid

HAN Ru-shuo; ZHENG Mei-ru; ZHANG Chen; RONG Chang; YAO Wei-yu; SHI Wan-yu; DU Xi-cui; WANG Xiao-dan

doi:10.13557/j.cnki.issn1002-2813.2023.23.017

Abstract：The study aimed to optimize the extraction and clarification process of Baitouweng oral liquid.The L9(34)orthogonal test was used to optimize the water extraction and clarification process of Baitouweng oral liquid.The extraction times were fixed for three times,the liquid to solid ratio,soaking time and extraction time were considered as factors,and the content of berberine hydrochloride was taken as evaluation index to explore the water extraction process.With the concentration of liquid,dosage of clarifying agent and flocculation temperature as factors,the absorbance and retention rate of berberine hydrochloride as comprehensive evaluation indexes,the clarifying process was explored and studied.At the same time,the flocculation clarifying process was compared with alcohol precipitation clarifying process.The results showed that the optimal water extraction process of Baitouweng oral liquid was as follows:liquid to solid ratio 10 mL/g,soaking time 30 min,extraction for three times,each extraction time 70 min.The optimal clarifying process was as follows:liquid concentration 1∶7 g/mL,dosage of ZTC1+1 natural clarifying agent 6%A and 3%B,flocculation temperature 60℃.The average retention rate of the berberine hydrochloride was 70.89%after flocculation and clarification process,while it was only 56.72%for berberine hydrochloride using the alcohol precipitation method.The relative standard deviation(RSDS)of content retention rate of the oral liquid prepared by flocculation and clarification from different batches was lower than 1.00%(n=3).The study indicates that the extraction and clarification process optimized in this experiment is stable and reliable.It can be used in industrial production.