Bioinformatics analysis of differentially expressed genes affected by selenium yeast in broiler liver based on GEO database
ZHANG Ji-qing
MA Xiu-liang
WANG Gui-ying
Abstract：The experiment was to screen differentially expressed genes(DEGs)in the livers of broilers supplemented with selenium yeast,to investigate the molecular mechanisms by which selenium yeast affects the growth,development,and health of broilers.The study obtained gene expression profile data from the Gene Expression Omnibus(GEO)and conducted bioinformatics mining.The results showed that 600 DEGs related to selenium yeast were identified.These DEGs were subjected to Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)annotation using DAVID software,leading to the significant enrichment of 18 biological processes and 12 KEGG pathways.A protein-protein interaction network(PPI)was constructed using the STRING database,which included 546 nodes and 905 edges.Using Cytoscape software,six hub genes(FN1,PLCG1,MYO3A,ITGA8,KDM6A,and ATP2B2)and four gene modules were identified.The study suggests that these six hub genes,along with the pathways related to the adrenergic signaling in the cardiomyocytes,calcium signaling,homologous recombination,focal adhesion,and eukaryotic ribosome biogenesis in myocardial cells,may be the key genes and key pathways through which selenium yeast influences the growth,development,and health of broilers.
Keywords：selenium yeastGEO databasebroiler liverdifferentially expressed genesbioinformatics analysis
Publication Date：2023-12-15
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 84-89 )