Study on effect of Sophora subprosrate polysaccharide on the inflammatory response and immunosuppression of immune cells in PCV2 infection through JAK-STAT/NF-κB signaling pathway
CHEN Qi
BAI Jing-jing
XIE Xiao-dong
ZHAO Yi
HU Ting-jun
Abstract：The study was to investigate the modulatory effect of Sophora subprosrate polysaccharide(SSP)on inflammatory reaction and immunosuppression in PCV2-infected RAW264.7 cells.The PCV2-infected RAW264.7 cells were treated with SSP(100,200 and 400 mg/L)for 16 h,the mRNA expression of signal transducer and activator of transcription 1,2(STAT1,STAT2),janus kinase 1(JAK1),tyrosine kinase 2(TYK2),chemokine(C-C motif)ligand 3(Ccl3),chemokine(C-X-C motif)ligand 2(CXCL2),tumor necrosis factor alpha(TNF-α),REL proto-oncogene,NF-κB subunit(Rel),nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor alpha and delta(Nfκbia,Nfκbid),and nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor zeta(Nfκbiz)were measured.The protein expression levels of phospho-signal transducer and activator of transcription 1 and 2(p-Stat1,p-Stat2),signal transducer and activator of transcription 1 and 2(Stat1,Stat2),inhibitor of nuclear factor κB kinase subunit beta(IκB),phospho-inhibitor of nuclear factor κB kinase subunit beta(p-IκB),nuclear factor NF-κB p65 subunit(p65),phospho-nuclear factor NF-κB p65 subunit(p-p65),CXCL2,JAK1,phospho-Janus kinase 1(p-JAK1)and TYK2 in the cells were detected.The levels of interleukin-1β(IL-1β),interferon-α(IFN-α),interferon-β(IFN-β),and TNF-α in cell supernatants were detected.The results showed that after the RAW264.7 cells were infected with PCV2 for 16 h,the mRNA expression levels of STAT1,STAT2,JAK1,TYK2 and Nfκbia were significantly upregulated(P＜0.05),mRNA expression levels of Ccl3,CXCL2,TNF-α,Rel,Nfκbid and Nfκbiz were significantly downregulated(P＜0.05),the protein expression levels of Stat1,p-Stat1,Stat2,p-Stat2,JAK1,p-JAK1 and TYK2 were significantly upregulated(P＜0.05),protein expression levels of IκB,p-IκB,p65 and CXCL2 were significantly downregulated in RAW264.7 cells(P＜0.05),and SSP at 200 mg/L significantly regulated the imbalance in the expression levels of these factors(P＜0.05).The study indicates that SSP modulates inflammatory response and immunosuppression induced by PCV2 infection through JAK-STAT/NF-κB signaling pathway.
Keywords：Sophora subprosrate polysaccharidePCV2inflammatory reactionimmunosuppressionsignaling pathway
Publication Date：2023-12-15
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：7( 67-73 )