Study on optimization and bioactivity of the fermentation medium for high yield exopolysaccharides of Schizophyllum commune
SONG Peng-wei
SUN Chang
DING Qiang
WANG Hong-lei
Abstract：The study used a single factor and orthogonal experiment design,to optimize the culture medium and study the biological activity of Schizophyllum commune exopolysaccharides.The results showed that the formula of the high yield exopolysaccharide medium was lactose 40 g/L,soybean peptone 8 g/L,3-indolebutyric acid 1 mg/L,KH2PO4 2 g/L,MgSO4·7H2O 1 g/L,pH value 6.0.After optimization,the polysaccharide yield reached(16.01±0.38)g/L,which was 3.03 times higher that of base medium.The IC50 value of exopolysaccharides for hydroxyl radical and DPPH free radical were 3.146 and 8.291 g/L,respectively.The IC50 value for Escherichia coli and Staphylococcus aureus were 3.516 and 9.732 g/L,respectively.At the RH of 43%,60%and 81%,the moisture absorbance rate of Schizophyllum commune exopolysaccharide within 60 h was 28.0%,42.0%and 50.9%,respectively,and the moisture retention rate within 48 h was 82.5%.The study indicates that the optimized medium formula can significantly increase the polysaccharide yield,and the Schizophyllum commune exopolysaccharide has a good antioxidant,antibacterial,hygroscopicity and moisture retention.
Keywords：Schizophyllum communeSchizophyllum commune exopolysaccharidefermentationantioxidationhygroscopicitymoisture retention
Publication Date：2023-11-30
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 86-91 )