Regulatory effect of Chinese herbal extract on lipopolysaccharide-induced mastitis in mice

LIU Yu-ran; YANG Hui-ying; WANG Meng-meng; ZHANG Meng-yuan; WANG Yan; LIAN Ke-xun; GU Xin-li

doi:10.13557/j.cnki.issn1002-2813.2023.22.015

Abstract：The aim of the experiment was to investigate the regulatory effect of Chinese herbal extract(ZY)on lipopolysaccharide-induced mastitis in mice.A total of 72 Kunming female rats with SPF grade 3～5 d postpartum were selected,12 were randomly selected as the blank control group(BC group),and the other 60 were randomly divided into five groups,namely the model group(M group),the positive control group(Dex group),the high dose(ZY-H group),the medium dose(ZY-M group)and the low dose(ZY-L group)of Chinese herbal extract,with 12 mice in each group.Mice in other groups except BC group were subjected to mastitis modeling after environmental adaptation.2 h after the modeling was completed,mice in the ZY-H group,ZY-M group and ZY-L group were given 16,8 and 4 mg/g Chinese herbal extract,respectively.Mice in the Dex group were injected with 5 mg/kg dexamethasone sodium phosphate injection peritoneally,and mice in the BC group and the M group were gavaged fed with normal saline of the same volume.After molding,it was treated continuously for 7 d.The results showed that the mammary gland acinar and acinar duct structures were destroyed and accompanied by inflammatory infiltration in the mammary gland tissue of mice in M group.Compared with the BC group,histopathological scores in the M group were extremely increased(P<0.01),the content of cytokines TNF-α,IL-1β,IL-6,IL-8 and MPO were extremely increased(P<0.01),and the content of PPAR-γ were extremely decreased(P<0.01).Compared with M group,the histopathological conditions of mammary gland in Dex group and ZY groups were recovered to a certain extent,the histopathological scores were extremely decreased(P<0.01),the content of TNF-α,IL-1β,IL-6,IL-8 and MPO were extremely decreased(P<0.01),and the content of PPAR-γ were extremely increased(P<0.01).The study indicates that each dose of Chinese herbal extract has a positive regulatory effect on LPS-induced mastitis in mice.