Effect of different target cell numbers on the cytotoxicity of NK-92MI against target cells with the same effector-to-target ratio
Pan Changqing
Qian Yuxing
Xu Menghui
Zhang Wei
Abstract：Objective To investigate the effect of different numbers of target cells on the cytotoxicity of NK-92MI against target cells at the same effector-to-target(E∶T)ratio and its potential underlying mechanisms.Methods Under standard 4-hour cytotoxicity conditions,NK-92MI cells were co-cultured with K562,BNI 2-4-S,BNI 1-3-S,and BNI 19-1-S cells in quantities of 20 000,10 000,5 000,and 2 500 cells,respectively,and the lysis ratio of target cells was detected to determine the cytotoxic efficacy of NK-92MI.NK-92MI was coupled with K562 and BNI 19-1-S cells in quantities of 2 500,5 000,10 000,and 20 000 to investigate the binding of NK-92MI to target cells.Results In the cytotoxicity assay,when the number of target cells was 2 500,5 000,10 000,and 20 000,the lysis ratios of NK-92MI co-cultured with K562 cells were(16.62±2.09)%,(20.84±1.36)%,(31.00±2.01)%,and(47.80±1.30)%,respectively;the lysis ratios of NK-92MI co-cultured with BNI 2-4-S cells were(29.94±2.27)%,(39.64±2.47)%,(49.29±1.27)%,and(64.25±1.92)%,respectively;the lysis ratios of NK-92MI co-cultured with BNI 1-3-S cells were(41.81±3.59)%,(48.59±3.41)%,(57.10±3.25)%,and(64.55±1.90)%,respectively;the lysis ratios of NK-92MI co-cultured with BNI 19-1-S cells were(41.39±2.39)%,(47.85±3.15)%,(54.09±0.67)%,and(57.82±2.48)%,respectively.The cytotoxic efficacy of NK-92MI increased with the number of target cells,with statistically significant differences(all P＜0.01).In the cell-coupling assay,when the target cell numbers were 2,500,5,000,10,000,and 20 000,the binding ratios of NK-92MI to K562 cells were(1.85±0.11)%,(5.30±0.37)%,(7.79±0.08)%,and(13.10±0.30)%,respectively.In the cell coupling assay,when the target cell numbers were 2 500,5 000,10 000,and 20 000,the binding ratios of NK-92MI to K562 cells were(1.85±0.11)%,(5.30±0.37)%,(7.79±0.08)%,and(13.10±0.30)%,respectively;and the binding ratios of NK-92MI to BNI 19-1-S cells were(2.58±0.21)%,(6.69±0.31)%,(8.46±0.20)%,and(13.37±0.78)%,respectively.As the number of target cells increased,the binding of NK-92MI to target cells significantly increased,with statistically significant differences(all P＜0.01).Conclusions At the same E∶T ratio,varying numbers of target cells influence the degree of contact between NK-92MI and target cells,thereby affecting the cytotoxic efficacy of NK-92MI against them.At the same E:T ratio,the greater the number of target cells,the more extensive the contact between NK-92MI and target cells,and the higher the cytotoxic efficacy of NK-92MI against them.
Keywords：NK-92MICytotoxicityCell couplingK562Glioma stem cells
Publication Date：2025-11-20
Online Publishing Date：2025-11-28(First online date of this platform, not the publication date of the document)
Pages：6( 798-803 )