Analysis of histopathological features and molecular pathological features of IDH wild-type glioblastoma
Liu Guoming
Xiao Menglin
Xu Can
Xu Jianglong
Tian Shaohui
Fang Chuan
Abstract：Objective To explore the histopathological and molecular pathological features of isocitrate dehydrogenase(IDH)wild-type glioblastoma(GBM).Methods From September 2021 to August 2023,74 GBM patients with surgery at the Department of Neurosurgery,Affiliated Hospital of Hebei University were selected as the study subject.Hematoxylin-eosin staining was used to observe the pathological characteristics of tissues.Immunohistochemical staining and next-generation sequencing were used to analyze molecular pathological features.Kaplan-Meier method was used to plot survival analysis curves to analyze the relationship between patient survival and IDH1 mutations.Results Among 74 cases of GBM,males accounted for 60.81%(45/74),and the age was(55.23±4.58)years old.Fifty-eight cases of tumors were located in the frontal lobe and 16 cases were located in the temporal lobe.The histological results of hematoxylin-eosin staining showed that 82.43%(61/74)of tumor tissues showed typical palisade necrosis,vascular proliferation,and significant tumor atypia,consistent with the typical GBM tissue morphological characteristics.The morphology of other tumor tissues matched the morphological characteristics of grade 2 or 3 astrocytoma.Immunohistochemical staining showed that all tumors were negative for IDH1 R132H staining,while Ki-67 staining showed an average positive rate of 38%.Results of the next-generation sequencing molecular pathology showed that all tissues were negative for IDH1/2,with positive amplification of epidermal growth factor receptor(EGFR)accounting for 74.32%(55/74)and telomerase reverse transcriptase(TERT)promoter region mutations accounting for 59.46%(44/74).The proportion of pure deletion of cyclin dependent kinase inhibitor 2A/B(CDKN2A/B)was 33.78%(25/74).The high expression level of methylation in the promoter region of O6-methylguanine-DNA methyltransferase(MGMT)accounted for 47.30%(35/74).Conclusions GBM exhibits molecular features of IDH wild-type,with some cases accompanied by EGFR amplification,TERT promoter region mutations,CDKN2A/B homozygous deletion,and MGMT promoter region hypermethylation.
Keywords：GlioblastomaPathologyIDH wild-typeHistopathologyMolecular pathology
Publication Date：2024-05-20
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 328-333 )