Effects of different stimulus parameters of repeatitive magnetic stimulation on expression of ndocannabinoid-related gene in cultured mice astrocytes

doi:10.3969/j.issn.1009-6574.2017.08.002

Abstract：Objective To explore the effects of different stimulus parameters of repetitive magnet-ic stimulation on the expression of endocannabinoid system-related genes, including cannabinoid receptor 1 (CB1R), diacylglycerol (DAGL), fatty acid amide hydrolase (FAAH) and monoacylglycerol (MAGL) in cultured mice astrocytes. Methods The astrocytes were isolated from the brain of newborn mice. After 7-10 d culture, the cells were purified and then treated with repeatitive magnetic stimulation with different stimulus parameters or control treatment. The magnetic stimulation group was divided into four groups: (1 Hz + 0.86T,1 Hz + 1.24 T, 5 Hz + 0.86 T) and (5 Hz + 1.24 T), which represented the stimulation including the magnetic frequency of 1 Hz or 5 Hz and the intensity of 0.86 or 1.24 Tesla. The control cells were placed in the same environment but 20 cm away from the magnetic field of the repeated magnetic stimulation group, and the magnetic field strength could not be detected. After the intervention, total mRNA and protein were extracted from the cells and the expression changes of CB1R, DAGL, FAAH and MAGL were detected. Results Compared with the control group, the expression levels of CB1R, MAGL and FAAH genes of astrocytes were significantly up-regulated when the cells were stimulated with (5 Hz + 0.86 T) magnetic stimulation. Meanwhile, the expression of FAAH gene were also up-regulated when the cells was stimulated with magnetic stimulation at (5 Hz + 1.24 T). However, there was no significant difference on the expression of DAGL between each group. Conclusions The effects of repeatitive magnetic stimulation of different parameters repeatitive magnetic stimulation induced different influence on the expression of endocannabinoid system-related gene in astrocytes, which might be involved in its different thera-peutic effects induced by different parameters.