Protective effect of bilobalide on cerebral ischemia reperfusion injury under acute hyperglycemia in rats
WANG Xu-gang
CAO Hua
Abstract：Objective To study the protective effect of bilobalide on cerebral ischemia reperfusion injury under hyperglycemia in rats and explore the possible mechanisms.Methods Rats were given 50% glucose(6 ml/kg) by intraperitoneal injection to get acute hyperglycemia model. Cerebral ischemia reperfusion model was made by intraluminal monofilament. 40 rats were randomly divided into hyperglycemia+sham opera-tion group(sham group,n=8), hyperglycemia+MCAO group(model group,n=8), hyperglycemia+MCAO+bilo-balide group(bilobalide groups,n=24). Bilobalide groups were then divided into three dose groups(2.5, 5 and 10 mg/kg,n=8). The bilobalide groups were given intraperitoneal injection for 3 d and 1 h before cerebral isch-emia. After blocking middle artery for 2 h and reperfusion for 24 h, neurological deficit scores, cerebral infarc-tion volume, brain water content, AQP4 mRNA, SOD activity, MDA content, NE, DA and 5-HT expression were detected.Results Rats in bilobalide(5, 10 mg/kg) group showed significantly relieved neurological deficit scores, reduced cerebral infarction volume, decreased brain water content, down-regulated AQP4 mRNA ex-pression, up-regulated SOD activity, reduced MDA content and improved content of NE, DA and 5-HT, com-pared to those in model group(P< 0.05).Conclusions Bilobalide could relieve cerebral ischemia reperfusion injury of cerebral ischemia reperfusion rats under acute hyperglycemia.
Keywords：BilobalideHyperglycemiaCerebral ischemia reperfusionNeuroprotection
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 420-423 )