Mechanism of therapeutic effects of intravenous transplantation of bone marrow derived mesenchymal stromal cells on ischemia in rats
Abstract：Objective To study effects of inflammatory cytokines and neurotrophic factors on intravenous transplantation of allogeneic bone marrow derived mesenchymal stromal cells (MSC) treatment of ischemia in rats.Methods The distal middle cerebral artery occlusion (dMCAO) was applied in this study by electrocoagulation.For the transplantation group,1 × 106 allogeneic MSC were intravascular transplanted into tail vein at 1 h after ischemia onset.The ischemia vehicle group received dMCAO surgery and intravascular saline injection.At 48 h after ischemia onset,the levels of pro-inflammatory cytokines (TNF-α,IL-1 β,IFN-γ,IL-6),anti-inflammatory cytokines (IL-4,IL-10),and neurotrophic factors (IGF-1,GDNF,BDNF) were measured in the ischemia cortex area and striatum by ELISA.Results At 48 h after ischemia onset,as compared with core area of ischemia vehicle group,allogeneic MSC decreased the levels of IFN-γ,IL-6 significantly,increased the levels of TNF-α,IL-1β,BDNF significantly.MSC also reduced the levels of IL-10,while increased IGF-1 significantly in striatum.No significant difference of GDNF was found between ischemia vehicle and MSC transplantation group.Conclusions Allogeneic MSC is beneficial to the ischemia damaged brain if transplanted at 1 h after the onset of ischemia.The effects of anti-inflammation are not the underlying mechanisms of the therapeutic effects at this time.That increased of IGF-1 and BDNF after MSC transplantation in the ischemia core of cortex area may contribute to the therapeutic effects of MSC transplantation group.
Keywords：Cerebral infarctionMesenchymal stem cellMicrogliaTransplantation
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 654-658,封3 )