Preliminary study on the comparative proteomics of the cerebellum of the rat depression model
Abstract：Objective To explore the role of cerebellum in the pathogenesis of depression,by analyzing the differently expressed proteins in the cerebellum of the rat depression model.Methods Chronic unpredictable mild stress (CUMS) rat depression model was conducted.Different cerebellum protein levels between CUMS and control rats were examined using a multi-dimensional liquid chromatographytandem mass spectrometry.The results were further analyzed with DAVID database.Results The CUMS rats showed decreased sucrose preference compared with the control rats (P ＜ 0.05).Among the 55 cerebellum proteins expressed differently between the CUMS and control rats,18 were down-regulated and 37 were up-regulated in the CUMS rats as compared with the control rats.These proteins were mainly associated with neurotransmitter metabolism,glycolysis,ATP metabolism,lipid metabolism,and amino acid metabolism.Conclusions The differentially expressed proteins found in this study may be the basis of the biological basis of depression,and provide important clues for the further study of the cerebellum role in the pathogenesis of depression.
Keywords：DepressionRatCerebellumProteomics
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 640-645 )