Effects of duloxetine and fluoxetine on S100B and ERK1/2-NF-κB expression in depression rats model

YANG Yuan; YU Xue; YANG Kun

doi:10.3969/j.issn.1009-6574.2016.05.006

Abstract：Objective To analyze the effects of duloxetine and fluoxetine on S100B and ERK1/2-NF-κB expression in hippocampus of depression rats model.Methods Chronic unpredictable mild stim-ulation was used to establish 54 depressive model rats.They were randomly divided into non-interven-tion group (B group,n =18),treatment with duloxetine group (C group,n =18)and treatment with fluoxetine group (D group,n = 18).Then 18 rats were selected as control group(A group).Behavior tests including the saccharine preference test and open- field test were used to measure the behavioral changes after 28 d intragastric administration.Western blot was used to detect S100B,ERK1/2 and NF-κB expression in hippocampus.Results In the saccharine preference test,rats in A,C and D group exhibited significantly increased saccharine preference [(63 ±1 1)%,(67 ±6)%,(61 ±10)%]compared to that in B group [(43±1 5)%]after 28 d intragastric administration (P < 0.05).In open-field test, the crossing score,rearing score and latency in C and D group were (70.66±1 1.53)and (67.54±10.08), (20.32±8.85)and (21.34±7.46),(1.0±0.4)s and (1.1±0.3)respectively,and showed no significant differences with A group [(71.1 9±12.08),(20.42 ±8.76)and (1.0 ±0.3)s]after 28 d intragastric ad-ministration (P > 0.05).While C and D group showed higher crossing score and rearing score,and shor-ter latency than B group.A,C and D group exhibited significantly increased expression of S100B, ERK1/2 and NF-κB than B group (P < 0.05),while there was no significant difference between C and D group.Conclusions Duloxetine and fluoxetine both can improve behavioral ability in depression rats and increase the expression of S100B and p-ERK1/2-NF-κB in hippocampus.There is no difference between the effect of duloxetine and fluoxetine on expression of related cytokines in depression rats.