Effects of AMP-activated protein kinase on microglia mediated inflammatory response in oxygen glucose deprivation/reperfusion injury
Abstract：Objective To study the effects of AMP -activated protein kinase(AMPK) on micro‐glia mediated inflammatory response in oxygen glucose deprivation/reperfusion injury (OGD/R) .Meth‐ods An in vitro ischemia model was induced by oxygen and glucose deprivation 6 h and reperfusion 24 h in BV2 cell . AMPK was activated and inhibited respectively by AICAR (5 μmol/L , 50 μmol/L , 100μmol/L ) and Compound C (0 .1μmol/L ,1μmol/L ,10μmol/L ) at different concentration .T he sur‐vival rate in BV2 cell after OGD/R was observed by MTT method .The phosphorylation levels of AMPK and IκB were detected by Western blot .The release of TNF -αwas detected by ELISA .Results Vari‐ous concentrations of AICAR were able to promote BV 2 cells survival (P < 0 .05) and inhibit the phos‐phorylation level of IκB (P < 0 .05)in OGD/R .AICAR (100μmol/L) could increase the phosphoryla‐tion level of AMPK (P< 0 .05) ,inhibit the release of TNF-α(P< 0 .01)in OGD/R .While Compound C (10μmol/L) promoted BV2 cell death (P< 0 .01) ,decreased the phosphorylation levels of AMPK and IκB (P < 0 .05) ,which could promote the release of TNF -α(P < 0 .05) .Conclusions Activation of AMPK by phosphorylation can relieve the inflammatory damage mediated by OGD/R in BV2 cells ,while inhibit the phosphorylation of AM PK can aggravate the inflammatory reaction .
Keywords：Oxygen glucose deprivation/reperfusion injuryAdenosine monophosphate-activa-ted protein kinaseMicrogliaInflammation
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 252-255 )