Mechanism of miR-181c inhibiting the invasion of glioblastoma cell line T 98G
Abstract：Objective To investigate the mechanism of miR -181c inhibiting the invasion of glio‐blastoma cell line T98G .Methods Using RT -PCR and Western Blot to detect the expression of miR -181c and TGF-βrelated genes .The effect of miR -181c and TGF -βon the invasion of glioma cells was measured by Transwell .Using molecular cloning technology to design and clone the target gene 3'-UTR and its mutation region .Using luciferase assay to analyse the combination of miR -181c and target genes .Results mRNA and protein expression of miR -181c and TGF -βpathway associated genes TG‐FBR1 ,TGFBR2 and TGFBRAP1 decreased (P< 0 .05) .miR-181c could weaken the invasion of T98G cells by influencing TGF -βsignal and the 3'-UTR of TGFBR1 ,TGFBR2 and TGFBRAP1 were direct targets of miR-181c .Conclusions miR -181c can inhibit the invasive ability of glioma cell line T 98G by regulating the TGF -βsignaling pathway ,and it can be an important molecule for glioblastoma treatment .
Keywords：GlioblastomaTransforming growth factor betaT98GInvasion
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 158-161 )