Protective effects of quetiapine on the lipopolysaccharide injured hippocampal -derived neural stem cell

doi:10.3969/j.issn.1009-6574.2016.02.002

Abstract：Objective To explore the effects of quetiapine(QUE) on viability and proliferation in LPS injured hippocampal -derived neural stem cells (NSCs) and investigate the role of ERK signal path‐way .Methods The NSCs were derived from hippocampus of fetal rats ,and divided into Sham group , LPS group and LPS+QUE group (including 0 .5 ,1 and 2μmol/L) .Then the cell viability ,the expres‐sion of pERK1/2 and BrdU labeling and detection were measured by the kit of WST -8 ,Western Blot and immunocytochemical method after 48 h ,respectively .Furthermore ,the cell viability and BrdU labe‐ling and detection were also detected after U 0126 treatment to clear the role of ERK signal pathway .Re‐sults (1)Cell viability test showed that the viability of LPS group [A= (0 .251 ± 0 .034) ] was signifi‐cantly lower than that of Sham group [A= (0 .371 ± 0 .027)] ,LPS+ QUE 1μmol/L group [A= (0 .311 ± 0.018)] and LPS+QUE 2 μmol/L group [A= (0 .343 ± 0 .021)] (P < 0 .05) .(2)The expression of pERK1/2 in LPS group (0 .313 ± 0 .124) was significantly lower than that of Sham group (1 .417 ± 0.141) ,LPS+QUE 1μmol/L group (0 .681 ± 0 .098) and LPS+QUE 2μmol/L group (0 .954 ± 0 .119) as detected by Western Blot .(3)The percentage of BrdU+ cells in LPS group (23 .098 ± 2 .153)% was significantly lower than that of Sham group (40 .388 ± 2 .910)% ,LPS+ QUE 1 μmol/L group (28 .742 ± 1 .536)% and LPS+QUE 2μmol/L group (31 .369 ± 2 .313)% .(4)The effects of QUE (1μmol/L and 2μmol/L) were inhibited by U0126 .Conclusions The damage of cell viability and proliferation of NSCs induced by LPS can be restrained by QUE ,and this effect might be related to the activation of ERK sig‐naling pathw ay .