Effects of Tanshinone ⅡA on the expression of GFAP and caspase-3 in the brain of experimental autoim- mune encephalomyelitis rats
YAN Jun
YANG Xue
FENG Juan
Abstract：Objective To observe the treatment effect of Tanshinone ⅡA on experimental autoim-mune encephalomyelitis rats and the expression of GFAP and caspase -3 in the brain.Methods Forty rats were randomly divided into four groups ,blank control group (Naive) ,high dose treatment group (TSⅡA -H) ,low dose treatment group (TSⅡA -L) and solvent control group (Vehicle).After in-duction of EAE model ,nerve function scores and weight were measured everyday.All the rats were kill-ed and drawn at the peak of the disease (the 18th day).HE statining and Lauck Fast Blue(LFB) staining were separately performed to observe the inflammatory infiltration and spinal cord demyelination situa-tion.The level of GFAP and caspase-3 in brain was detected via immunohistochemistry and immunobo-ltting (western blot).Results Compared to the Vehicle group rats ,better clinical symptoms ,less infil-tration of inflammatory cells and lower levels of GFAP and caspase-3 in brain were found in both trea-ted groups.Conclusions Tanshinone Ⅱ A had therapeutic effect on EAE rats.This might be due to down-regulation of GFAP and caspase-3 ,which participates in the regulation of inflammatory reaction and apoptosis process.
Keywords：Multiple sclerosisCaspase-3Experimental autoimmune encephalomyelitisTanshinone ⅡAGFAP
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 50-54 )