Protective effect of Saikosaponin B2 on damage of cultured SH-SY5Y cells in vitro introduced by hydrogen peroxide
Abstract：Objective To investigate the effect of Saikosaponin B2 on the damage of cultured SH -SY5Y cells. Methods Damaged SH -SY5Y cells model was established with the method of cell culture in vitro. 10% calf serum including volume fraction 0. 05 ,0. 10 ,0. 20 Saikosaponin B2 (10-4 mol/L) were added respectively into the SH -SY5Y cells ,which were then treated with 140μmol/L hydrogen perox‐ide (H2 O2 ). 10% calf serum group and blank serum without H2 O2 -treated group were as the model group and the control group. The effect of Saikosaponin B2 was observed by morphological identification , colorimetric MTT assay. Results Both Saikosaponin B2 of 1 × 10-6 mol/L and 2 × 10-6 mol/L can relieve the damage of SH -SY5Y cells and increase the survival of the cells. Conclusions Saikosaponin B2 can protect the cultured SH -SY5Y cells from damage induced by H2 O2.
Keywords：Saikosaponin B2MTTSH -SY5Y cellsMorphologyHydrogen peroxide
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 221-224 )