An Experimental Study on Implantation of Microencapsulated PC12 Cells for Treatment of Parkinson's Disease
Abstract：Objective To investigate a newer method for treatment of Parkinson's disease (PD).Methods A newer method of immunoisolation (microencapsulation) was utilized for encapsulation of dopamine-secreting PC12 cells, and the cells were implanted into the brains of Parkinson's disease rats.And then, the change of apomorphine-induced rotational behavior in PD rats were observed. The quantities of dopamine (DA) in the area of transplantation were evaluated by high performance liquid chromatography with electrochemical detection (HPLC-EC). Tyrosine hydroxylase (TH) antibody immunohistochemistry stains of the cells in the implanted area were also proceeded. Results The apomorphineinduced rotational behavior of PD rats reduced significantly, the quantities of DA in the implanted area were increased much more than the control area at the different times. At the same time, the lethal tumor formation was observed in unencapsulated PC12 cells implantation. The microcapsules in the PD rats brains were intact and TH immunopositive PC12 cells could exist for 3 months postimplantation.Conclusions Implantation of microencapsulated PC12 cells for treatment of Parkinson's disease may be a new and effective method that can avoid the shortcoming of agents, stereotactic therapy, and implantation of embryonic brains.
Keywords：Microencapsulation PC12 cell Implantation Parkinson's disease
Publication Date：2003-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：2( 181-182 )