Identification of Key Hub Genes in Infiltrating Lymphocytes of Esophageal Carcinoma Based on Weighted Gene Co-expression Network
HOU Yongjie
CAO Heng
YANG Haijun
ZHOU Fuyou
Abstract：Objective To use Weighted Gene co-expression Network Analysis(WGCNA)identifing key genes associated with esophageal cancer and provide data support for related research,in order to iden-tify the gene expression of the infiltrating lymphocyte antigen receptor region in esophageal cancer.Meth-ods Tissue samples of 70 esophageal cancer patients from our hospital was selected and gene sequenced,meanwhile,transcriptome data were downloaded from NCBI SRA database.The co-expression network of e-sophageal cancer was constructed by R WGCNA,and the co-expression modules that most strongly correlated with esophageal squamous cell carcinoma were screened out.The hub genes were analyzed by Centiscape.Results The results of network analysis indicate that MEgrey60、MEpurple and MEblack modules were highly correlated with esophageal cancer.According to protein-protein interaction,the top 20 hub genes of each module were selected.The biological function of the genes in the module are mainly in-volved in endopeptidase inhibitor activity,specific RNA polymerase Ⅱ transcription factor activity,histone binding,antioxidant activity,thioredoxin peroxidase activity,ATPase activity and protein transporter activi-ty.Conclusion The key genes of infiltrating lymphocytes in esophageal cancer identified in this study can provide targets and theoretical basis for the diagnosis,treatment,and immunotherapy of esophageal cancer.
Keywords：esophageal cancerWGCNAtargethub genetumor-infiltrating lymphocytes
Publication Date：2024-06-30
Online Publishing Date：2026-09-12(First online date of this platform, not the publication date of the document)
Pages：7( 141-147 )