Effects of Versican on Migration and Invasion of Esophageal Squamous Cell Carcinoma Cell in Vitro

ZHOU Dongli; LIU Jiaxing; ZHANG Feng; ZHANG Weiguo

doi:10.15926/j.cnki.issn2096-7381.2024.01.003

Abstract：Objective To explore the expression of Versican(VCAN)in esophageal squamous cell carcinoma(ESCC)KYSE150 and EC9706 cells and its impact on cell migration and invasion in vitro.Methods The GSE77861 data set was downloaded from the GEO database,bioinformatics methods were used to process the data and screen differentially expressed genes,and the key gene VCAN related to the prognosis of ESCC was screened out.Western blot was used to detect the protein expression levels of VCAN in human normal esophageal epithelial cells HEEC and four ESCC cell lines KYSE70,KYSE140,KYSE150,and EC9706.The treated KYSE150 and EC9706 cells were divided into 3 groups:si-VCAN silent expression group,si-NC negative control group and blank control group.Western blot was used to detect the protein ex-pression level after silent expression of VCAN,wound healing assay was used to detect the effect of silent ex-pression of VCAN on cell migration ability,and Transwell assay was applied to detect the effect of silent expression of VCAN on cell invasion capacity.Re-sults Western blot results showed that the relative expression level of VCAN in ESCC cell lines was higher than that in human normal esophageal epithe-lial cells HEEC.The si-VCAN silent expression group's VCAN expression was less than that of the si-NC negative control and the blank control group(P<0.05).The wound healing and transwell assay results showed that the number of migrating and in-vading cells in the si-VCAN silenced expression group was reduced compared to the control group(P<0.05).Conclusion VCAN is highly expressed in ESCC cell lines,and silencing the expression of VCAN can inhibit the migration and invasion of ESCC cells in vitro.