Virulence factors FimA of Porphyromonas Gingivalis Inhibits the Immunity by Triggering MHC-I Degradation
ZHANG Xudong
ZHANG Xiusen
YANG Ze
CHEN Lele
DING Jianlan
YUAN Xiang
Abstract：Objective To investigate the impact and regulatory mechanisms of Pg infection on MHC-I molecules in esophageal squamous cell carcinoma(ESCC)cells.Methods Western blot was employed to assess the expression levels of the major virulence factor fimbriae(FimA)and MHC-I after Pg infection of KYSE-150 cells.Immunoprecipitation(Co-immunoprecipitation,Co-IP)was used to explore the interaction between FimA and MHC-I.Confocal microscopy was utilized to monitor changes in fluorescence following LC3 dual-fluorescence lentivirus transduction in KYSE150 cells.Western blot was conducted to measure the expression of LC3B-Ⅱ after Pg infection in KYSE150 cells.Flow cytometry was employed to evaluate Ki67 expression in Peripheral Blood Mononuclear Cells(PBMCs)following co-culture with Pg-infected KYSE150 cells.Results Western blot revealed increased expression of FimA and decreased expression of MHC-I after Pg infection of KYSE150 cells.Co-IP indicated a direct interaction between FimA and MHC-I proteins.Confocal microscopy demonstrated enhanced fluorescence intensity after Pg infection of KYSE150 cells.Flow cytometry showed significant reduction of Gzmb and Mki67 expression in PBMCs co-cultured with Pg-infected KYSE150 cells(both P<0.001).Conclusion The Pg virulence factor FimA may downregulate MHC-I expression through the autophagy pathway and suppress T-cell immune responses,thereby promoting the formation of an inhibitory immune microenvironment in ESCC and facilitating tumor progression.
Keywords：esophageal squamous cell carcinomaPorphyromonasgingivalisautophagyvirulence factortumor immune microenvironment
Publication Date：2024-03-30
Online Publishing Date：2026-09-12(First online date of this platform, not the publication date of the document)
Pages：7( 1-7 )