Diagnostic and prognostic value of serum lncRNA ZFAS1 and lncRNA HOTAIR in patients with polycystic ovary syndrome
ZHENG Xue
ZENG Qingwei
CHEN Xiaoyu
BIAN Xuehua
Abstract：Objective To investigate the predictive value of serum long non-coding RNA zinc finger protein anti-sense chain 1(lncRNA ZFAS1)and long non-coding RNA homologous box gene transcription antisense RNA(lncRNA HOTAIR)in the early diagnosis and prognosis of patients with polycystic ovary syndrome(PCOS).Methods Totally 198 PCOS patients(research group)and 198 healthy women of reproductive age who underwent physical examination dur-ing the same period(control group)were selected.Fasting venous blood was drawn from the subjects of the two groups.Real-time fluorescent quantitative PCR(qRT-PCR)was used to detect serum lncRNA ZFAS1 and lncRNA HOTAIR.Pa-tients received the standardized treatment for 3 months,and the prognosis was determined based on the ultrasound re-exam-ination results.Multivariate Logistic regression was applied to analyze the factors affecting the prognosis of PCOS.Receiv-er operating characteristic(ROC)curve was used to evaluate the value of serum lncRNA ZFAS1 and lncRNA HOTAIR in the diagnosis and prognosis prediction of PCOS.Results Compared with the control group,the research group had high-er relative expression levels of serum lncRNA ZFAS1 and lncRNA HOTAIR(both P<0.05).Among 198 patients with PCOS,there were 107 patients with good prognosis and 91 patients with poor prognosis.Compared with patients with poor prognosis,patients with good prognosis had lower relative expression levels of serum lncRNA ZFAS1 and lncRNA HO-TAIR(both P<0.05).Multivariate Logistic regression showed that elevated levels of serum lncRNA ZFAS1,lncRNA HO-TAIR and AMH were risk factors affecting the prognosis of PCOS patients(all P<0.05),and elevated levels of FSH and ES were protective factors affecting the prognosis of PCOS patients(both P<0.05).ROC curve showed that the area under the curve(AUC)of serum lncRNA ZFAS1,lncRNA HOTAIR alone and in combination for the diagnosis of PCOS were 0.712,0.750,and 0.813,respectively.The AUC for predicting poor prognosis of PCOS using serum lncRNA ZFAS1 and lncRNA HOTAIR alone and in combination were 0.765,0.819,and 0.867,respectively.Conclusions The serum lev-els of lncRNA ZFAS1 and lncRNA HOTAIR in patients with PCOS are elevated.The levels of serum lncRNA ZFAS1 and lncRNA HOTAIR have certain value in the early diagnosis and poor prognosis evaluation of PCOS.
Keywords：polycystic ovary syndromelong non-coding RNA zinc finger protein antisense chain 1long non-cod-ing RNA homologous box gene transcription antisense RNA
Publication Date：2025-12-25
Online Publishing Date：2026-01-12(First online date of this platform, not the publication date of the document)
Pages：6( 81-85,90 )