Impact and mechanism of recombinant IL-35 administered via tail vein injection on renal ischemia-reperfusion injury in mice
SUN Qing
LI Wenwen
FAN Li
JI Xiaobing
Abstract：Objective To investigate the effect of tail vein injection of recombinant interleukin-35(pIL-35)on re-nal ischemia-reperfusion injury(IRI)in mice and to explore its potential mechanisms.Methods Forty-two C57BL/6J mice were randomly divided into the sham-operated group(sham group,n=6),model group(n=18),and pIL-35 group(n=18).Mice in the pIL-35 group received the tail vein injection of recombinant pIL-35(50 μg)plus 2 mL PBS,while those in the sham and model groups were given the empty vector(50 μg)plus 2 mL PBS.After injection,renal IRI models were established in the model and pIL-35 groups by clamping both renal pedicles for 40 min followed by 1 min reperfusion.The sham group underwent identical procedures without vascular clamping.Serum IL-35,creatinine(Cr),and blood urea nitrogen(BUN)levels were measured by ELISA before intervention and on day 1,3,and 5 after intervention.Renal tis-sue IL-16 and tumor necrosis factor-α(TNF-α)levels were determined by ELISA on day 1,3,and 5,and the expression of Janus kinase 2(JAK2)/signal transducer and activator of transcription 3(STAT3)pathway-related proteins was ana-lyzed by Western blotting.Results Compared with pre-intervention values,serum IL-35,Cr,and BUN levels increased in both the model and pIL-35 groups after intervention;however,the pIL-35 group showed significantly higher IL-35 and lower Cr and BUN levels than the model group at all time points(all P<0.05).Compared with the sham group,the expres-sion levels of IL-16,TNF-α,and JAK2/STAT3 pathway proteins were significantly elevated in the model and pIL-35 groups on day 1,3,and 5,while the expression levels of IL-16,TNF-α and the JAK2/STAT3 signaling pathway protein were lower in the pIL-35 group than in the model group at the same time point(all P<0.05).Conclusion Tail vein in-jection of pIL-35 can alleviate renal ischemia-reperfusion injury in mice,possibly by regulating the JAK2/STAT3 signaling pathway and reducing inflammatory responses.
Keywords：interleukin-35ischemia-reperfusion injuryacute kidney injuryJAK2/STAT3 signaling pathwayinflammatory response
Publication Date：2025-12-25
Online Publishing Date：2026-01-12(First online date of this platform, not the publication date of the document)
Pages：5( 41-45 )