Application value analysis of thyroid biopsy combined with BRAF V600E gene test in the diagnosis of papillary thyroid carcinoma
WU Zhaoyi
OUYANG Huiting
LI Zhecheng
FAN Peizhi
Abstract：Objective To analyze the application value of thyroid biopsy combined with BRAF V600E gene detec-tion in the diagnosis of papillary thyroid carcinoma(PTC).Methods A retrospective study was conducted on 293 pa-tients with thyroid nodules who underwent thyroid surgery.Preoperative thyroid biopsy and BRAF V600E gene testing were performed,and intraoperative pathological results were used as the gold standard.A paired four grid table was used to eval-uate the diagnostic efficacy of thyroid biopsy,BRAF V600E gene testing,and their combination for PTC.Logistic regres-sion was used to construct a diagnostic PTC model combining biopsy and BRAF V600E gene testing.Results Among 293 patients in the intraoperative pathological diagnosis,there were 283 cases of PTC and 10 cases of non-PTC.Among 283 pathologically diagnosed PTC patients,241 cases were diagnosed with PTC and 42 cases were diagnosed with non-PTC through thyroid biopsy,with a positive rate of 85.61%;the BRAF V600E gene test results showed 280 cases of PTC and 3 cases of non-PTC,with a positive rate of 98.94%in biopsy.Compared with thyroid biopsy,BRAF V600E gene detection in diagnosis of PTC had a higher positive rate(P<0.05).The accuracy,sensitivity,and specificity of thyroid biopsy in di-agnosing PTC were 85.7%,85.2%,and 100%,respectively;the accuracy,sensitivity,and specificity of BRAF V600E gene testing for diagnosing PTC were 99.0%,98.9%,and 100%,respectively;the accuracy,sensitivity,and specificity of the combined detection and diagnosis of PTC were 100%,100%,and 100%,respectively.The diagnostic model of PTC using biopsy combined with BRAF V600E was Logit(P/1-P)=-0.147+1.165×biopsy smear results+1.431×BRAF V600E gene detection results.The total score of the LogP type combined model of biopsy smear and BRAF V600E gene de-tection in diagnosing PTC was 181 points.Conclusion The combination of thyroid biopsy and BRAF V600E gene test-ing has high diagnostic value in PTC.
Keywords：thyroid tumorpapillary thyroid carcinomapuncture biopsyBRAF genegene test
Publication Date：2025-12-25
Online Publishing Date：2026-01-12(First online date of this platform, not the publication date of the document)
Pages：4( 31-34 )