Therapeutic effect and mechanism of Adropin on myocardial fibrosis in rats with diabetic cardiomyopathy
CHEN Pengfei
WANG Siliang
ZHOU Can
ZENG Gang
LYU Zhan
LIU Mao
Abstract：Objective To investigate the therapeutic effects of Adropin(ADR)on myocardial fibrosis in rats with di-abetic cardiomyopathy(DCM),and to explore its potential mechanisms.Methods Fifteen 8-week-old male SD rats were randomly divided into three groups:normal control group(CON group),high-glucose group(DCM group),and ADR intervention group(ADR group),with 5 rats in each group.The DCM models were established in the DCM group and ADR group through intraperitoneal injection of 1%streptozotocin,while the CON group received no treatment.After modeling,the ADR group was intraperitoneally injected with ADR at a dose of 10 μg/100 g,and the DCM group and CON group were intraperitoneally injected with normal saline at 1 mL/100 g,once daily for 8 consecutive weeks.After sacrific-ing the rats,myocardial tissues were collected to calculate the ratio of left ventricular weight(LVW)to body weight(LVW/BW),to observe pathological changes,and to detect the expression levels of inflammatory factors[tumor necrosis factor-α(TNF-α)and interleukin-6(IL-6)],myocardial fibrosis markers[collagen type Ⅰ(Col Ⅰ)and collagen type Ⅲ(ColⅢ)],transforming growth factor-β1(TGF-β1)/Smad signaling pathway proteins[TGF-β1,Smad3,phosphorylated Smad3(phos-Smad3)],and p38 mitogen-activated protein kinase(p38)signaling pathway-related proteins[p38,phosphorylat-ed p38(phos-p38)].Results Compared with the CON group,the LVW,LVW/BW,and the expression levels of TNF-α,IL-6,Col Ⅰ,Col Ⅲ,TGF-β1,phos-p38,and phos-Smad3 in the DCM group significantly increased(all P<0.05).After ADR intervention,compared with the DCM group,the LVW,LVW/BW,and the expression levels of TNF-α,IL-6,Col Ⅰ,Col Ⅲ,TGF-β1,phos-p38,and phos-Smad3 in the ADR group significantly decreased(all P<0.05).Conclu-sion Adropin alleviates myocardial fibrosis in DCM rats,potentially by down-regulating the activity of p38 MAPK and TGF-β1/Smad signaling pathway.
Keywords：energy homeostasis-regulating peptideAdopindiabetic cardiomyopathymyocardial fibrosisp38 mitogen-activated protein kinasetransforming growth factor-β1Smad3
Publication Date：2025-12-25
Online Publishing Date：2026-01-12(First online date of this platform, not the publication date of the document)
Pages：5( 11-14,18 )