Effect and mechanism of intraperitoneal injection of seaweed polysaccharide combined with budesonide nasal spray on allergic rhinitis rats
ZHOU Mingyu
WANG Xin
JI Wenjun
LI Jing
GAO Dongsheng
ZHANG Tiantian
Abstract：Objective To observe the therapeutic effect of intraperitoneal injection of seaweed polysaccharide com-bined with budesonide nasal spray on allergic rhinitis(AR)rats,and to explore its possible mechanism.Methods Twen-ty-five SPF healthy SD rats were randomly divided into the normal control group,model control group,budesonide group,seaweed polysaccharide group,seaweed polysaccharide+budesonide group,with 5 mice in each group.In addition to the normal control group,the AR models were established by intraperitoneal injection of ovalbumin(OVA)as a sensitizing agent and nasal instillation of OVA nasal stimulant.After modeling was successfully completed,the budesonide group re-ceived nasal spray administration of budesonide,while the seaweed polysaccharide group received intraperitoneal injection of 200 mg/kg seaweed polysaccharide.The seaweed polysaccharide+budesonide group received both nasal spray and in-traperitoneal injection.The normal control group and model control group received the equal volume of normal saline solu-tion via nasal spray and intraperitoneal injection.After 2 weeks of treatment,the nasal symptoms and signs were observed and scored in each group.ELISA was used to detect serum interleukin-33(IL-33)and growth-stimulated expression gene 2(ST2),and real-time fluorescent quantitative PCR was used to detect IL-33 and ST2 mRNA expression in the nasal mu-cosa tissues.Results Rats in the normal control group did not have allergic rhinitis symptoms such as sneezing,runny nose,etc.,and occasionally scratched their noses;rats in the model control group successively showed the phenomenon of nose scratching and sneezing,and finally showed obvious nose scratching,sneezing and clear runny nose after stimula-tion;rats in the budesonide group,seaweed polysaccharide group,and seaweed polysaccharide+budesonide group had fewer nose scratches,sneezing and runny nose than rats in the model control group.Compared with the normal control group,the scores of nasal symptoms and signs,serum IL-33 and ST2 levels,and the relative expression levels of IL-33 and ST2 mRNA in nasal mucosa all increased in the model control group(all P<0.05).Compared with the model control group,the scores of nasal symptoms and signs,serum IL-33 and ST2 levels,and the relative expression of IL-33 and ST2 mRNA in nasal mucosa decreased in the budesonide group,seaweed polysaccharide group and seaweed polysaccharide+budesonide group,and the decrease was more obvious in the seaweed polysaccharide+budesonide group(all P<0.05).Conclusion The combination of seaweed polysaccharide intraperitoneal injection and budesonide nasal spray may re-duce the nasal symptoms of AR rats by reducing the level of IL-33 and ST2 in serum and nasal mucosa.
Keywords：allergic rhinitisseaweed polysaccharideinterlenkin-33growth-stimulated expression gene 2
Publication Date：2025-10-25
Online Publishing Date：2025-11-20(First online date of this platform, not the publication date of the document)
Pages：4( 49-52 )