Effect and mechanism of ethyl acetate extract of Blaps rynchopetera Fairmaire on malignant biological behavior of human ovarian cancer cells
CUI Xia
LU Huixia
Abstract：Objective To explore the impacts of the ethyl acetate extract of Blaps rynchopetera Fairmaire(BRF)on the proliferation,migration,invasion,and autophagy of human ovarian cancer SKOV3 cells.Methods SKOV3 cells in the logarithmic growth phase were divided into the extract group,cisplatin group,hydroxychloroquine group,and rapamy-cin group,which were added 2 mL each of 10 μg/mL BRF extract,5 mg/L cisplatin,60 μmol/L autophagy inhibitor hy-droxychloroquine,and 200 μg/L mammalian target of rapamycin(mTOR)inhibitor rapamycin,respectively.Cell prolifer-ation was detected through the colony formation assay.Cell migration was detected by Scratch assay.Cell invasion was de-tected using Transwell chamber assay.The expression levels of matrix metalloproteinases(MMPs)family members MMP2,MMP9,and autophagy-related proteins[microtubule-associated protein 1 light chain 3(LC3),mTOR]were de-tected by Western blotting.Results Compared with the control group,the number of cell colonies formed,cell migra-tion rate,and and the number of invasive cells were lower in all treated groups(all P<0.05).Among these,the extract group had the smallest number of cell colonies formed,the lowest cell migration rate,and the fewest invasive cells(all P<0.05).Compared with the control group and the extract group,the cisplatin group and the hydroxychloroquine group had higher expression levels of MMP2 protein(all P<0.05);there was no statistically significant difference in the MMP2 pro-tein expression between the extract group and the control group(P>0.05).Compared with the control group and the cispla-tin group,the expression levels of MMP9 protein were lower in the extract group,the rapamycin group,and the hydroxy-chloroquine group(all P<0.05).Compared with the control group,the expression levels of mTOR protein and LC3Ⅱ/LC3Ⅰ were higher in the extract group,rapamycin group,and hydroxychloroquine group(all P<0.05),with the ex-tract group having the highest relative expression level of mTOR protein and the lowest LC3II/LC3I ratio(both P<0.05).Conclusion BRF extract could inhibits SKOV3 cell proliferation,migration,and invasion,which may be related to down-regulating MMP9 expression and activating mTOR signaling pathway to induce cell autophagy.
Keywords：ovarian carcinomaethyl acetate extract of Blaps rynchopetera Fairmairecell proliferationcell migra-tioncell invasioncell autophagy
Publication Date：2025-08-25
Online Publishing Date：2025-09-10(First online date of this platform, not the publication date of the document)
Pages：5( 1-5 )