Analysis of mechanism of action of Kushen Zhiyang powder in the treatment of allergic conjunctivitis
ZHAO Fan
JIANG Peiying
YU Jingsheng
GAO Chunting
MENG Diao
Abstract：Objective To explore the mechanism of action of Kushen Zhiyang powder in the treatment of allergic conjunctivitis(AC).Methods The active ingredients and target genes of each herb of Kushen Zhizhi powder were screened using the Traditional Chinese Medicine System Pharmacology Analysis Platform(TCMSP),and the target genes acting on humans were retrieved from the Uniprot database.We screened the disease target genes for AC through Gene-Cards,OMIM,and drugbank databases(revised as disease target genes for AC in full text).Screening the intersection tar-get genes of Kushen Zhizhi powder and AC target genes through Venn diagram,and constructing a protein interaction net-work diagram through STRING database to screen the key target genes of Kushen Zhiyang powder for treatment of AC.We performed Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)enrichment analysis on key tar-get genes using the Metascape database.Molecular docking of core target genes and active ingredients of Kushen Zhiyang powder was performed using Autodock software.Results There were 97 active ingredients and 228 target genes in Kush-en Zhiyang powder,and 2,349 disease target genes in AC.There were 156 intersecting target genes for the treatment of AC with Kushen Zhiyang powder.The key target genes for the treatment of AC with Kushen Zhiyang powder mainly includ-ed protein kinase Bα(AKT1),nuclear factor κB1A(NFκB1A),and mitogen-activated protein kinase 1(MAPK1).Kushen Zhiyang powder mainly affected the response of AC cells to external stimuli,membrane rafts,and transcription fac-tor binding through pathways such as phosphatidylinositol 3-kinase protein kinase B(PI3K-AKT)signaling pathway,NF-κB signaling pathway,and Janus kinase/signal transducer and activator of transcription(JAK-STAT)signaling pathway,etc.The active ingredients of Kushen Zhiyang powder,such as coralline and hesperetin,bound to the core protein AKT1 in a sta-ble conformation.Conclusion Kushen Zhiyang powder may exert therapeutic effects on AC by regulating the gene expres-sion of AKT1,NFκB1A,and MAPK1,as well as the PI3K-AKT,NF-κB,JAK-STAT and other signaling pathways.
Keywords：Kushen Zhiyang powderallergic conjunctivitisnetwork pharmacologymolecular docking
Publication Date：2025-05-25
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 36-40 )