Identification of volatile ingredients in ethyl acetate,petroleum ether and chloroform extracts of Cremastra appendiculata

CHU Yumeng; LI Dijia; LI Yajing; YAO Fang; ZHANG Ziying; GAO Ruixia

doi:10.3969/j.issn.1002-266X.2024.36.009

Abstract：Objective To identify the volatile ingredients in ethyl acetate,petroleum ether and chloroform extracts of Cremastra appendiculata.Methods Three kilograms of Cremastra appendiculata were processed into powder and ex-tracted with ethyl acetate,petroleum ether and chloroform,respectively.The volatile ingredients in ethyl acetate extract,petroleum ether extract and chloroform extract were analyzed and identified by gas chromatography-mass spectrometry(GC-MS).Results Fifty-one ingredients were identified from the ethyl acetate extract of Cremastra appendiculata,with the highest content of catechol,followed by 4-hydroxybenzaldehyde,2-ethyl-3-methylpyrazine,Phenol,4-(ethoxymethyl)-,cinnamic acid,m-hydroxybenzoic acid,etc.Thirty-five ingredients were identified from the petroleum ether extract of Cremastra appendiculata,including cinnamic acid,butyl palmitate,methyl heptadecanoate,oleic acid,linoleic acid,etc.Twenty-eight ingredients were identified from the chloroform extract of Cremastra appendiculata,including Phenol,4-(eth-oxymethyl)-,2,6-dimethoxyphenol,2-ethyl-3-methylpyrazine,cinnamic acid,etc.Conclusion Cinnamic acid was found in the volatile ingredients of ethyl acetate,petroleum ether and chloroform extracts of Cremastra appendiculata.Anti-cancer ingredients such as vanillic acid,m-hydroxybenzoic acid and 4-hydroxybenzaldehyde were found in the ethyl acetate extract of Cremastra appendiculata.Catechol,2-ethyl-3-methylpyrazine,Phenol,3-methylcyclopentane-1,2-dione,meth-ylcyclopentenolone,butyl palmitate,methyl heptadecanoate,oleic acid,linoleic acid,2,6-dimethoxyphenol,2-ethyl-3-methylpyrazine and other ingredients were found in different extracts of Cremastra appendiculata for the first time.