Inhibitory effect of ginsenoside Rg1 on hypoxia-induced human retinal pigment epithelial cell injury and its mechanism
BAI Mei
MIAO Deyu
WU You
Abstract：Objective To explore the effect and mechanism of ginsenoside Rg1(GRg1)on hypoxia-induced human retinal pigment epithelium(RPE)cell injury.Methods ARPE-19 cells were cultured in vitro and randomly divided into control group,model group,high-dose GRg1 group,medium-dose GRg1 group,low-dose GRg1 group,and hypoxia-in-duced factor 1α(HIF-1α)inhibitor group,respectively.Except for the control group,ARPE-19 cells in the other groups were induced by cobalt chloride to construct anoxic model.CCK-8 method and TUNEL method were used to determine cell activity and apoptosis in each group at different time points.The levels of reactive oxygen species(ROS)in each group were measured by DCFH-DA fluorescent probe,and HIF-1α,brain-derived neurotrophic factor(BDNF)and pituitary ade-nylate cyclase activating polypeptide(PACAP)38 mRNA and protein expression levels in each group were measured by RT-qPCR and Western blotting.Results Compared with the control group,the other groups had significantly lower ARPE-19 cell activity and significantly higher apoptosis rates at 0,12 and 24 h after administration(all P<0.05).At 12,24 and 48 h after administration,the activity of ARPE-19 cells in the high-dose GRg1 group was obviously higher than that in the model group and low-dose GRg1 group(both P<0.05),while the apoptosis rate was obviously lower than those in the model group and low-dose GRg1 group(both P<0.05);the cell activity in the high-dose GRg1 group was significantly higher than that in the medium-dose GRg1 group at 24 and 48 h after administration(both P<0.05).Compared with the control group,the other groups had significantly higher ROS and HIF-1α mRNA and protein expression levels in ARPE-19 cells at 48 h after administration(all P<0.05).In the high-dose GRg1 group,the expression levels of ROSHIF-1α mRNA and protein in ARPE-19 cells were obviously lower than those in the model group and low-dose GRg1 group(all P<0.05),while BDNF,PACAP38 mRNA and protein expression levels in ARPE-19 cells were significantly higher than those in the model group and low-dose GRg1 group(all P<0.05).There were no statistically significant differences in cell activity,apoptosis rate,ROS,HIF-1α,BDNF or PACAP38 expression levels between high-dose GRg1 group and HIF-1α inhibitor group at different time points(all P>0.05).Conclusion GRg1 can alleviate the hypoxia-induced ARPE-19 cell injury,and its mechanism may be related to inhibiting the expression levels of ROS and HIF-1α,and up-regulating the expression levels of BDNF and PACAP38.
Keywords：retinal pigment epithelial cellshypoxiaginsenoside Rg1brain-derived neurotrophic factorpitu-itary adenylate cyclase-activating polypeptide 38
Publication Date：2024-12-05
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 29-33 )