Expression changes of miR-145-5p and IGF2BP1 mRNA in endometrial cancer tissues and their relationship with the prognosis
TANG Yaonan
HE Aiqin
SHAO Jia
ZHANG Can
Abstract：Objective To investigate the expression changes of microRNA-145-5p(miR-145-5p)and insulin-like growth factor 2 mRNA-binding protein 1(IGF2BP1)in the endometrial carcinoma(EC)tissues and their relationship with the prognosis of patients.Methods Totally 115 specimens of EC tissues and corresponding adjacent tissues surgically resected in Nantong Tumor Hospital from May 2018 to May 2021 were selected,and real-time fluorescence quantitative PCR was used to detect the expression of miR-145-5p and IGF2BP1 mRNA in EC tissues and adjacent tissues.Pearson correlation was used to analyze the correlation between miR-145-5p and IGF2BP1 mRNA expression.According to the median miR-145-5p and IGF2BP1 mRNA expression in EC tissues,we divided the patients into the high/low expression groups.Kaplan-Meier method was used to plot the survival curves of EC patients with high/low miR-145-5p and IGF2BP1 mRNA expression;multifactor Cox regression was used to analyze the factors affecting the prognosis of EC patients.Results Compared with the adjacent tissues,miR-145-5p expression decreased and IGF2BP1 mRNA expression increased in EC tissues(t=-22.201,19.038,both P<0.05).The miR-145-5p was negatively correlated with IGF2BP1 mRNA expression(r=-0.748,P<0.05).In the EC tissues,miR-145-5p and IGF2BP1 mRNA expression was related to degree of differentiation,federation international of gynecology and obstetrics(FIGO)staging,and lymph node metastasis(all P<0.05).The 3-year overall survival rate of 115 EC patients was 67.83%(78/115).Patients with high miR-145-5p expression had a higher 3-year overall survival rate than patients with low miR-145-5p expression,and the 3-year overall survival rate of patients with high expression of IGF2BP1 mRNA was lower than that of patients with low expression of IGF2BP1 mRNA(χ2=13.484,11.755,all P<0.05).The independent risk factors for death in EC patients were poor dif-ferentiation,FIGO stage III,lymph node metastasis,and IGF2BP1 mRNA≥1.53(OR=5.434,6.092,4.327,and 5.339;95%CI:1.735-17.021,1.930-19.225,1.424-13.154,and 1.515-18.811),and the independent protective factor was miR-145-5p≥0.74(OR=0.298,95%CI:1.515 to 18.811).Conclusion Low expression of miR-145-5p and high expression of IGF2BP1 mRNA in EC tissues were associated with the degree of differentiation,FIGO stage,lymph node metastasis and prognosis.
Keywords：endometrial carcinomamicroRNA-145-5pinsulin-like growth factor 2 mRNA-binding protein 1pathological parametersprognosis
Publication Date：2024-12-05
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 15-19 )