Screening and analysis of key differentially expressed genes in pericoronal adipose tissues of patients with coronary artery disease complicated with type 2 diabetes mellitus
HU Yahui
ZHANG Linxin
CHEN Ying
LI Yan
XING Yan
Abstract：Objective To analyze the differentially expressed genes in pericoronary adipose tissues(PCAT)of pa-tients with coronary artery disease(CAD)combined with type 2 diabetes mellitus(T2DM),to screen the key differentially expressed genes of CAD and T2DM,and to validate the findings.Methods In the GEO database,the chip datasets GSE64554,GSE120774,GSE16415,and GSE71416 were obtained.Genes that were differentially expressed in both CAD and T2DM were screened out for functional annotation and pathway analysis to identify key genes associated with CAD and T2DM.Twenty patients undergoing heart valve replacement surgery,20 CAD patients,and 20 CAD patients with T2DM were included in the control group,CAD group,and CAD+T2DM group,respectively.PCAT samples collect-ed during surgery were analyzed for key differentially expressed genes using RT-qPCR.Results A total of 54 differential-ly expressed genes were identified in the PCAT of CAD and T2DM patients.Functional annotation revealed three key genes:major histocompatibility complex class II DRβ1(HLA-DRβ1),tumour protein 53(TP53),and C-C motif chemo-kine receptor 9(CCR9).The relative expression levels of TP53 gene increased sequentially in the control group,CAD group,and CAD+T2DM group(all P<0.05).The expression of CCR9 in the CAD and CAD+T2DM groups was higher than that in the control group(both P<0.01).The expression of HLA-DRβ1 in the CAD+T2DM group was higher than in the control group(P<0.05).Conclusions The key genes in the PCAT of CAD patients with T2DM are HLA-DRβ1,TP53,and CCR9,with significant expression changes in the TP53 gene,which may contribute to the diagnosis of CAD complicated with T2DM.
Keywords：coronary artery diseasetype 2 diabetes mellituspericoronal adipose tissuesTP53 geneHLA-DRβ 1 geneCCR9 genebioinformatics analysis
Publication Date：2024-11-15
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 11-15 )