Expression changes of LncRNA-WT1-AS and LncRNA-PRRT3-AS1 in NSCLC tissues and their predictive efficacy for prognosis
GONG Cheng
GAO Mingyue
MENG Junhong
PENG Cong
ZHANG Xiaoya
XU Jian
LIU Duxian
Abstract：Objective To observe the expression changes of long non-coding RNA(LncRNA)-WT1-AS and Ln-cRNA-PRRT3-AS1 in non-small-cell lung cancer(NSCLC)tissues,and to explore their predictive efficacy for prognosis.Methods A total of 158 patients with NSCLC confirmed by pathological examination were selected.All patients under-went tumor resection.During the operation,NSCLC cancer tissues and adjacent tissues were collected.LncRNA-WT1-AS and LncRNA-PRRT3-AS1 were detected by real-time fluorescence quantitative PCR.The relationships between LncRNA-WT1-AS,LncRNA-PRRT3-AS1 and clinicopathological parameters were analyzed.Kaplan-Meier method was used to draw the 3-year survival curves of NSCLC patients with high and low expression of LncRNA-WT1-AS and LncRNA-PRRT3-AS1.Multivariate COX regression analysis was used to analyze the influencing factors of poor prognosis of NSCLC pa-tients.The receiver operating characteristic(ROC)curve was drawn to analyze the predictive efficacy of LncRNA-WT1-AS and LncRNA-PRRT3-AS1 expression on the poor prognosis of NSCLC.Results Compared with the adjacent tissues,the relative expression of LncRNA-WT1-AS was lower,and the relative expression of LncRNA-PRRT3-AS1 was higher in the cancer tissues(both P<0.05).The proportion of high expression of LncRNA WT1-AS in NSCLC patients with tumor diameter≥3 cm,poor differentiation,TNM stage Ⅱ,and less lymph node metastasis was low(χ2=7.680,4.300,13.830,and 4.135,respectively;P<0.05);the proportion of high expression of LncRNA-PRRT3-AS1 was higher in NSCLC patients with tumor diameter≥3cm,poor differentiation,TNM stage Ⅱ,and more lymph node metastasis was high(χ2=8.072,3.898,14.833,and 10.040,respectively;P<0.05).Compared with those with low expression of Ln-cRNA-WT1-AS,those with high expression of LncRNA-WT1-AS had a higher 3-year survival rate(Log-rank=9.191,P<0.05).Compared with those with high expression of LncRNA-PRRT3-AS1,those with low expression of LncRNA-PRRT3-AS1 had a higher 3-year survival rate(Log-rank=8.783,P<0.053).Tumor diameter≥3 cm,poor differentiation,lymph node metastasis,TNM stage Ⅱ,low expression of LncRNA-WT1-AS and high expression of LncRNA-PRRT3-AS1 were independent risk factors for poor prognosis of NSCLC patients(all P<0.05).The area under the curve of LncRNA-WT1-AS and LncRNA-PRRT3-AS1 in predicting poor prognosis of NSCLC was 0.767(95％CI:0.714-0.820)and 0.848(95％CI:0.807-0.890),respectively;the cut-off values were 2.810 and 2.705,the sensitivities were 53.8％and 72.8％,and the specificities were 99.4％and 84.8％,respectively.Conclusions LncRNA-WT1-AS is low expressed and LncRNA-PRRT3-AS1 is highly expressed in NSCLC tissues.The prognosis of NSCLC patients with low expression of LncRNA-WT1-AS and high expression of LncRNA-PRRT3-AS1 is poor.The sensitivity and specificity of low expression of LncRNA-WT1-AS and high expression of LncRNA-PRRT3-AS1 in predicting poor prognosis of NSCLC are high.
Keywords：long non-coding RNAlong non-coding RNA-WT1-ASlong non-coding RNA-PRRT3-AS1non-small-cell lung cancer
Publication Date：2024-10-25
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 5-9 )