Effects of knocking down coenzyme Q10B on proliferation,apoptosis and EMT of esophageal squamous cell carcinoma xenografts in nude mice
WEI Yu
LI Zhifang
CAO Leiyu
GAO Yan
MA Xiaoli
ZHANG Li
Abstract：Objective To investigate the effects of coenzyme Q10B(COQ10B)knockdown on the proliferation,apoptosis and epithelial-mesenchymal transition(EMT)of esophageal squamous cell carcinoma xenografts in nude mice.Methods The interference lentivirus of COQ10B and its negative control were transfected into KYSE150 cells by lentivi-rus transfection technology.The KYSE150 cells with knockdown of COQ10B expression(sh-COQ10B group)and the nega-tive control KYSE150 cells(sh-NC group)were constructed.Twenty female BALB/c nude mice were randomly divided in-to the sh-COQ10B group(n=10)and sh-NC group(n=10).KYSE150 cells transfected with sh-COQ10B and sh-NC were subcutaneously injected into the right anterior scapula of nude mice to establish subcutaneous transplantation tumor mod-els.The growth of subcutaneous xenografts at different time after inoculation was observed and the size and the weight of tu-mors were measured.At the end of the experiment on the 26th day after inoculation,tumor tissues were taken,HE,immu-nohistochemistry,and TUNEL staining methods were used to observe cell proliferation and apoptosis in subcutaneous xeno-grafts in nude mice.Western blotting was used to detect the expression levels of EMT-related proteins(E-cadherin and Vi-mentin)and apoptosis-related proteins(Bcl-2 and Bax).Results Compared with the sh-NC group,the protein expres-sion of COQ10B in the sh-COQ10B group decreased(P<0.05).The subcutaneous transplanted tumor models were estab-lished successfully and no death occurred in nude mice.The tumor volume and mass in the sh-COQ10B group were lower than those in the sh-NC group(both P<0.05).Compared with the sh-NC group,the apoptosis rate of tumor cells in the sh-COQ10B group increased,the protein expression levels of Bcl-2 and Vimentin in tumor tissues decreased,and the expres-sion levels of Bax and E-cadherin increased,with statistically significant difference(all P<0.05).Conclusion Knock-ing down COQ10B expression can inhibit the proliferation of subcutaneous transplanted tumor and the occurrence of EMT in nude mice,and promote the apoptosis of tumor cells.
Keywords：esophageal squamous cell carcinomaxenografts in nude micecoenzyme Q10Bcell proliferationapoptosisepithelial-mesenchymal transition
Publication Date：2024-10-05
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 40-43 )