Expression of PGC1α and FNDC5 proteins in ovarian tissues of rats with letrozole-induced polycystic ovary syndrome
WANG Xinyan
XIAO Chengwei
LIANG Lin
Abstract：Objective To investigate the expression of peroxisome proliferator-activated receptor γ co-activator 1α(PGC-1α),and fibronectin type Ⅲ domain-containing protein 5(FNDC5)in the ovarian tissues of rats with letrozole-in-duced polycystic ovary syndrome(PCOS).Methods Six-week-old female SD rats were randomly divided into the con-trol group and PCOS group,with 10 rats in each group.Rats in the PCOS group were given letrozole solution by gavage to establish the PCOS models,and rats in the control group were given an equal volume of carboxymethyl cellulose solution by gavage,once a day,for 28 consecutive days.Chemiluminescence method was used to measure serum androgen(T),lu-teinizing hormone(LH),follicular estrogen(FSH),and fasting glucose(FPG);ELISA was used to measure fasting insu-lin(INS),and homeostatic model assessment for insulin resistance(HOMA-IR)was calculated;HE staining was used to observe the pathological changes of ovarian tissues;immunohistochemical staining and Western blotting were used to de-tect the protein expression levels of PGC-1α and FNDC5.Results There was no significant difference in serum FPG be-tween the two groups(P>0.05),the serum levels of T,LH,LH/FSH,INS,HOMA-IR and FNDC5 in the PCOS group were higher than those in the control group(all P<0.05),and the serum levels of FSH and PGC-1α were lower than those in the control group(all P<0.05).In the control group,follicles and fresh corpus luteum at different growth stages were visible,and the granulosa cells were neatly and tightly arranged,with a large number of layers,up to 6-8 layers.In the PCOS group,multiple immature cystic follicles and atresia follicles were seen,and the granulosa cells were arranged irreg-ularly with a small number of layers.PGC-1α and FNDC5 proteins were mainly expressed in granulosa cells and cumulus cells,showing a brownish-yellow color,while follicular membrane cells were expressed in small amounts.Compared with the control group,the expression of PGC-1α protein in the ovarian tissues was lower and the expression of FNDC5 protein was higher in the PCOS group,and the difference was statistically significant(all P<0.05).Conclusion PGC-1α was low expressed and FNDC5 was highly expressed in the ovarian tissues of PCOS rats.
Keywords：polycystic ovary syndromeperoxisome proliferator-activated receptor γco-activator 1αfibronectin type III domain-containing protein 5letrozolerats
Publication Date：2024-07-05
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 49-52 )