Effects and mechanism of miR-125a on biological behaviors of endometrial cancer cells
HE Jianqing
YANG Lifen
YIN Yue
CHEN Ying
Abstract：Objective To investigate the effects of microRNA-125a(miR-125a)on the proliferation,apoptosis,mi-gration,invasion,and other biological behaviors of endometrial cancer(EC)cells and the mechanism.Methods Hu-man EC cells(JEC,HEC-1B,and Ishikawa cells)and endometrial epithelial cells(ECS cells)were cultured routinely.The expression of miR-125a in cells was detected by RT-PCR,and the cells with the lowest expression of miR-125a were screened as experimental cells.EC cells in the logarithmic growth phase and with the lowest expression of miR-125a were taken and randomly divided into miR-NC group and miR-125a mimics group,which were transfected with miRNA negative control miR-NC and miR-125a mimics,respectively.The expression of miR-125a was detected by RT-PCR,the cell prolif-eration activity(OD value)was detected by CCK-8 assay,the cell colony formation ability was detected by plate cloning as-say,the apoptosis ability(apoptosis rate)was detected by flow cytometry,the cell migration and invasion capacities were detected by Transwell chamber assay,and the intracellular LIN28B protein expression was detected by Western blotting.Results Compared with ECS cells,the relative expression levels of miR-125a in JEC,HEC-1B and Ishikawa cells were lower(all P<0.05),and the relative expression of miR-125a in Ishikawa cells was the lowest,which were used as experi-mental cells.Compared with the miR-NC group,the expression of miR-125a in the miR-125a mimics group was higher(P<0.05).Compared with the miR-NC group,the OD values of miR-125a mimics group were lower after culture for 24,48,and 72 h,and the number of colony formation was smaller,the apoptosis rate was higher,the migrating cells and invasive cells were less,and the relative expression of LIN28B protein was lower,and the differences were statistically significant(all P<0.05).Conclusion Up-regulation of miR-125a expression inhibits the proliferation,migration and invasion of EC cells and induces apoptosis,which may be related to its targeted regulation of LIN28B gene.
Keywords：endometrial carcinomamicroRNA-125alin-28 homolog Bcell proliferationapoptosiscell migra-tioncell invasion
Publication Date：2024-07-05
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 45-48 )