Effects of isorhynchophylline on inflammation,apoptosis and ERK signaling pathway of acute pancreatitis cells in rats
ZHAI Xu
PEI Honghong
LIU Jun
HUANG Wan
Abstract：Objective To observe the effects of isorhynchophylline(LSO)on inflammation,apoptosis and ERK sig-naling pathway of acute pancreatitis(AP)cells in rats.Methods AR42J cells were divided into six groups.Cells in the control group were cultured normally;cells in the model group,LSO group,inhibitor group,LSO+ inhibitor group and LSO+ activator group were added with 100 nmol/L cerulein to prepare AP cell models,and cells in the LSO group were added with 40 μmol/L LSO after modeling,10 μmol/L U0126 was added to the inhibitor group,40 μmol/L LSO and 10 μmol/L U0126 were added to the LSO+ inhibitor group,and 40 μmol/L LSO and 0.1 μmol/L C16-PAF were added to the LSO+ activator group.Inflammatory factors(TNF-α,IL-6,IL-8,IL-1β),proliferation rate,and apoptosis rate in cell cul-ture medium were determined by ELISA,EdU method and Hoechst 33258 staining,respectively.The mRNA levels of nu-cleotide oligomerization domain-like receptor protein 3(NLRP3),speckle-like protein(ASC)and Caspase-1 in the cells were determined by qPCR,and the protein levels of ERK 1/2,p-ERK 1/2,NLRP3,ASC and Caspase-1 were determined by WB.Results Compared with the control group,TNF-α,IL-6,IL-8,IL-1β,apoptosis rate,p-ERK 1/2 protein,NL-RP3,ASC,Caspase-1 mRNA and protein increased,and the proliferation rate decreased in the model group(all P<0.05).Compared with the model group,inflammatory cytokines(TNF-α,IL-6,IL-8,IL-1β),apoptosis rate,p-ERK 1/2 protein,NLRP3,ASC,Caspase-1 mRNA and protein decreased,and proliferation rate increased in the LSO group and inhibitor group(all P<0.05).Compared with the LSO group,U0126 in the LSO+ inhibitor group enhanced the effect of LSO on the above trend of cells,while C16-PAF in the LSO+ activator group reversed the effect of LSO on the above trend of cells(all P<0.05).Conclusion LSO can inhibit the inflammation and apoptosis of AP cells probably by regulating ERK signaling pathway.
Keywords：isorhynchophyllineacute pancreatitisinflammationcell proliferationapoptosisnucleotide oligo-merization domain-like receptor protein 3 inflammasomeextracellular signal-regulated kinase pathway
Publication Date：2024-04-25
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 15-19 )