Effects of Danggui Shaoyao San-medicated serum and its active ingredients on renal interstitial fibrosis rats
LIU Huajun
ZHANG Ting
Abstract：Objective To investigate the interventional effects of Danggui Shaoyao San(DSS)-medicated serum and its active ingredients ferulic acid(FA)and paeoniflorin(PF)on renal interstitial fibrosis(RIF)rats and the mecha-nism.Methods DSS solution was prepared and administered to male Sprague Dawley rats by gavage at the same time of the day at low(0.05 mL DSS solution/10 g body mass),medium(0.1 mL DSS solution/10 g body mass),and high(0.2 mL DSS solution/10 g body mass)doses for 7 d.Blood was obtained from the abdominal aorta to obtain the equiva-lent low-,medium-,and high-dose of DSS serum(DSSS).Rat renal tubular epithelial cells(NRK-52E)were divided in-to the control group(Control),the model group(treated with TGF-β),the siRNA transfection group(treated with TGF-β +siRNA),the FA group(treated with TGF-β+FA),the PF group(treated with TGF-β+PF),and the low-,medium-,and high-dose DSSS groups(treated with TGF-β+DSS-L,TGF β+DSS-M,and TGF-β+DSS-H),respectively.The inter-vention concentrations of DSSS,FA,PF and TGF-β on NRK-52E cells were screened and determined for NRK-52E cells in the above groups.Western blotting was used to detect the changes in the protein levels of NLRP3,cysteine protease 1(CASP1),Gasdermin D(GSDMD),interleukin-18(IL-18),fibronectin(FN),E-Cadherin,and N-Cadherin in each group.Results Compared with the model group,the expression levels of pyroptosis-related proteins NLRP3,CASP1,GSDMD,and IL-18 decreased in the DSSS,FA,and PF groups,and the difference was statistically significant(all P<0.05).Compared with the model group,the expression levels of fibrosis-related adhesion proteins FN,E-Cadherin and N-Cadherin decreased in the medium-dose and high-dose DSSS and FA and PF groups,and the difference was statistically significant(all P<0.05).Conclusion DSS-medicated serum and its active ingredients,paeoniflorin and ferulic acid,can inhibit the activation of NLRP3-associated pyroptosis proteins in the TGF-β-induced fibrosis model of NRK-52E cells and regulate the expression of adhesion proteins and the progression of epithelial-mesenchymal transdifferentiation to allevi-ate the fibrosis.
Keywords：chronic kidney diseaserenal interstitial fibrosisDanggui Shaoyao Sanpyroptosisrenal tubular ep-ithelial cells
Publication Date：2024-04-05
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 36-40 )