Effects of METTL14 on proliferation,apoptosis,invasion and migration of acute promyelocytic leukemia cells
ZHANG Mengmeng
WU Yanping
ZHANG Pingping
WANG Meng
LI Jiajia
Abstract：Objective To investigate the effects of overexpression and knockdown of methyltransferase-like protein 14(METTL14)on proliferation,apoptosis,invasion and migration of acute promyelocytic leukemia cells.Methods Human acute promyelocytic leukemia cells NB4 were cultured,and were divided into the overexpression control(Over-NC)group,overexpression group,interference control(si-NC)group,and interference group,respectively.The blank plasmid and METTL14 plasmid were transfected into NB4 cells in the control group and the overexpression group,respec-tively.The si-NC and si-METTL14 were transfected into NB4 cells in the interference control group and the interference group,respectively.Cell proliferation was detected by CCK-8,apoptosis was detected by flow cytometry,cell invasion and migration abilities were detected by Transwell chamber,and N-6 methyladenine(m6A)modification level was detect-ed by Dot blotting assay.Results Compared with the Over-NC group,the cell proliferation rate increased,apoptosis rate decreased,cell migration number and cell invasion number increased,and m6A modification level of NB4 cells in-creased in the overexpression group(all P<0.05).Compared with the si-NC group,the proliferation rate decreased,apop-tosis rate increased,cell migration number and cell invasion number decreased,and the m6A modification level of NB4 cells decreased in the interference group(all P<0.05).Conclusion METTL14 can promote the proliferation,migration and invasion of NB4 cells,and its mechanism may be related to the increase of m6A modification level.
Keywords：acute promyelocytic leukemiamethyltransferase-like protein 14cell proliferationapoptosiscell invasioncell migrationmethyltransferaseN-6 methyladenine
Publication Date：2024-01-15
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 27-30 )