Therapeutic effect and mechanism of intragastric administration of Jiangtang Shuxin recipe suspension on diabetic heart failure in rats

JIANG Xiaofeng; CHEN Jiajun; HUANG Guangming; SHEN Yaya; FU Xianzhao

doi:10.3969/j.issn.1002-266X.2023.36.010

Abstract：Objective To observe the therapeutic effect of intragastric administration of Jiangtang Shuxin recipe on diabetic heart failure(DHF)in rats and to explore its mechanism.Methods Fifty SD rats were randomly divided into five groups,with 10 rats in each group.DHF models were prepared in the low-dose group,high-dose group,Western med-icine group,and model group except the control group.Rats in the low-dose,high-dose groups were given 1.0 and 1.5 g/(kg·d)Jiangtang Shuxin recipe suspension by gavage,respectively.Rats in the Western medicine group were given gliq-uidone and benazepril by gavage for 2 months,and were fed with high-fat diet.Rats in the control group were fed with ordi-nary diet.Fasting blood glucose(FBG),serum triglyceride(TG),low-density lipoprotein cholesterol(LDL-C),high-den-sity lipoprotein cholesterol(HDL-C),C-reactive protein(CRP),interleukin-6(IL-6),tumor necrosis factor-α(TNF-α),creatine kinase isoenzyme(CK-MB),lactate dehydrogenase(LDH),pathological morphology of myocardial tissues,NF-κB p65 protein and IκBα protein were compared among groups.Results Compared with the control group,the levels of FBG,serum TG,LDL-C,CRP,IL-6,TNF-α,CK-MB and LDH increased,the level of serum HDL-C decreased,the myocardial tissue was seriously damaged,the expression of NF-κB p65 protein increased,and the expression of IκBα protein decreased in the other four groups(all P＜0.05).Compared with the model group,the levels of FBG,serum TG,LDL-C,CRP,IL-6,TNF-α,CK-MB and LDH decreased,the serum HDL-C level increased,the myocardial tissue damage was alleviated,the expression of NF-κB p65 protein decreased,and the expression of IκBα protein increased in the low-dose group,the high-dose group and the Western medicine group(all P＜0.05).Compared with the Western med-icine group,the levels of FBG,serum TG,LDL-C,CRP,IL-6,TNF-α,CK-MB and LDH decreased,and the level of se-rum HDL-C increased in the high-dose group(all P＜0.05).Conclusions Jiangtang Shuxin recipe has a therapeutic ef-fect on DHF in rats,with the best effect in the high-dose group.It can not only alleviate high glucose and high fat state,but also reduce myocardial injury and inflammation,and improve the pathological morphology of myocardial cells.The mechanism may be related to its inhibition of NF-кB signaling pathway.