Expression changes of hsa_circ_0131457 in pancreatic cancer tissues and the effect of its overexpres-sion on cell biological behavior

KONG Panpan; DONG Yongliang; LIU Huifang; YAN Dong

doi:10.3969/j.issn.1002-266X.2023.35.003

Abstract：Objective To observe the expression changes of hsa_circ_0131457 in the pancreatic cancer tissues,and to investigate the effects of hsa_circ_0131457 overexpression on the proliferation,apoptosis,migration,invasion and other biological behaviors of pancreatic cancer cells.Methods Seven cases of pancreatic cancer tissues and 7 cases of matched normal adjacent normal tissues were collected from pancreatic cancer cells PANC-1,SW1990,CFPAC-1,and normal pancreatic duct epithelial cells HPDE6-C7 in the logarithmic growth phase.The expression of hsa_circ_0131457 was detected by real-time fluorescence quantitative PCR.The pancreatic cancer cells with the lowest expression of hsa_circ_0131457 were divided into the overexpression group and control group,which were transfected with hsa_circ_0131457 over-expression plasmid and control plasmid by liposome transfection method,respectively.After transfection,OD values of cells in the two groups were detected by CCK-8 method at 0,24,48,72 and 96 h,apoptosis rate was detected by TUNEL staining method,cell migration distance was recorded by cell scratch test,and the number of transmembrane cells was re-corded by Transwell chamber test.The relative expression levels of apoptosis-related protein cyclin D2(CCND2)and cy-clin dependent kinase 4(CDK4)were detected by Western blotting.Results The relative expression levels of hsa_circ_0131457 in the pancreatic cancer tissues and adjacent normal tissues were 0.011±0.017 and 2.084±2.287,with statisti-cally significant difference(P＜0.05).The relative expression levels of hsa_circ_0131457 in PANC-1,SW1990 and CF-PAC-1 cells were 0.123±0.110,0.159±0.082 and 0.141±0.129,respectively;all of them were lower than that of nor-mal pancreatic ductal epithelial cells(1.174±0.839)(all P＜0.05).Therefore,the pancreatic cancer cells PANC-1 with the lowest expression of hsa_circ_0131457 was used for follow-up study.There were no significant differences in OD values between the two groups at 0 and 24 h(all P＜0.05),and the OD values of the overexpression group were lower than those of the control group at 48,72 and 96 h(all P＜0.05).The apoptosis rate of the overexpression group was higher than that of control group,the cell migration distance was shorter and the number of transmembrane cells was smaller than those of the control group(all P＜0.05).The relative expression levels of CCND2 and CDK4 protein in the overexpression group were higher than those in the control group(all P＜0.05).Conclusions The expression of hsa_circ_0131457 decreases in both pancreatic cancer tissues and cells.Overexpression of hsa_circ_0131457 can inhibit the proliferation,migration and inva-sion of pancreatic cancer cells,and promote apoptosis by up-regulating the expression of CCND2 and CDK4.