Effect and mechanism of miR-214 on proliferation,apoptosis,and cell cycle of multiple myeloma cells

SHENG Xiuyun; ZHANG Lei; WANG Yanjun

doi:10.3969/j.issn.1002-266X.2023.34.010

Abstract：Objective To investigate the effects of expression of microRNA-214(miR-214)on the proliferation,apoptosis,and cell cycle of multiple myeloma(MM)cells and its mechanism.Methods The expression of miR-214 in MM cell lines IM-9,U266,Lp-1,H929 and human normal plasma cells was detected by quantitative real-time fluores-cence PCR(qRT-PCR).U266 cells were randomly divided into the miR-214 mimics group and mimics-NC group.MiR-214 mimics and mimics-NC were transfected into U266 cells of the two groups,respectively.The expression differences of miR-214 between the two groups after transfection were detected by qRT-PCR;the differences in colony formation num-ber,OD value at 24,48,72 and 96 h,apoptosis rate,and cell cycle proportion between the two groups were detected by plate cloning experiment,CCK-8 experiment,and flow cytometry,respectively;and the expression difference of FOXM1 protein between the two groups after transfection was detected by Western blotting.Results The expression levels of miR-214 in the IM-9,U266,Lp-1,and H929 cells were significantly lower than that in the normal plasma cells(all P<0.05).After transfection,compared with the mimics-NC group,the expression level of miR-214 in U266 cells in the miR-214 mimics group significantly increased(P<0.05),the number of colony formation significantly decreased(P<0.05),and the OD value significantly decreased at 24,48,72,and 96 h(all P<0.05),the rate of apoptosis and the proportion of G0/G1 in the cell cycle significantly increased(P<0.05),the proportion of S phase and G2/M phase significantly de-creased(P<0.05),and the expression level of FOXM1 protein significantly decreased(P<0.05).Conclusions The miR-214 is low expressed in MM cells,and up-regulation of miR-214 inhibits the proliferation of MM cells and promotes their apoptosis,blocking the cell cycle at the G0/G1 phase.The mechanism may be achieved by inhibiting the expression of FOXM1 gene.