Effect and mechanism of brexpiprazole on cholesterol synthesis of human colorectal cancer cell lines HCT116 and SW620
LI Ting
LONG Xiaoyi
LIU Xiaojie
CHEN Wei
Abstract：Objective To observe the effect of brexpiprazole on cholesterol synthesis in colorectal cancer cell lines HCT116 and SW620,and to explore its mechanism.Methods HCT116 and SW620 cells in the logarithmic growth phase were divided into the brexpiprazole group and control group,respectively.Cells in the brexpiprazole group were add-ed with medium containing 20 μmol/L brexpiprazole,while cells in the control group were added with equal amount of me-dium.After the cells were cultured for 24 h,the total cholesterol(TC)in the cells was detected by microplate assay.Hu-man 3-hydroxy-3-methylglutaryl-CoA reductase(HMGCR)mRNA and human 3-hydroxy-3-methylglutaryl-CoA synthase 1(HMGCS1)mRNA were detected by real-time fluorescence quantitative PCR.HMGCR,HMGCS1,p-PI3K,p-AKT,and SREBP2 proteins were detected by Western blotting.Results The TC levels of HCT116 and SW620 cells were lower in the brexpiprazole group than in the control group(both P<0.05).The relative expression levels of HMGCR mRNA and HMGCS1 mRNA in HCT116 and SW620 cells in the brexpiprazole group were lower than those in the control group(all P<0.05).The relative protein expression levels of HMGCR,HMGCS1,p-PI3K,p-AKT and SREBP2 in HCT116 and SW620 cells of the brexpiprazole group were lower than those of the control group(all P<0.05).Conclusion Brexpipra-zole inhibits intracellular cholesterol synthesis in HCT116 and SW620 cells possibly by inhibiting the protein expression of PI3K/Akt-SREBP2 signaling pathway.
Keywords：brexpiprazolecolorectal cancer cellstotal cholesterolPI3K/Akt-SREBP2 singling pathway
Publication Date：2023-11-25
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 7-10 )