Expression of TWEAK,Fn14 and NF-κ B pathway in PBMC of patients with senile sarcopenia in agricultural and pastoral areas of Xinjiang

ZHAO Yanjiao; LIU Jinling; Zhuoya Maimaitiwusiman; FENG Zhiqun; YANG Xingyu; WANG Hongmei

doi:10.3969/j.issn.1002-266X.2023.32.006

Abstract：Objective To observe the expression of tumor necrosis factor-like weak inducer of apoptosis(TWEAK),fibroblast growth factor-induced early reactive protein 14(Fn14)and nuclear factor-κB(NF-κB)in peripher-al blood mononuclear cells(PBMC)of patients with senile sarcopenia(later referred to as sarcopenia)in agricultural and pastoral areas of Xinjiang,and to explore their significance.Methods Eighty participants were selected from the perma-nent residents in agricultural and pastoral areas of Xinjiang.Forty patients with sarcopenia were included in the sarcopenia group and 40 healthy people were included in the control group.PBMC was extracted from fasting peripheral blood of the two groups,and TWEAK,Fn14,inhibitor of IκB kinase-α(IKKα),inhibitor of IκB kinase-β(IKKβ)and NF-κB p65 mRNA were detected by qRT-PCR.Results The mRNA expression levels of TWEAK,Fn14,IKKα and IKKβ in PBMC of the sarcopenia group were higher than those of the control group(all P<005).After adjusting for sex and age,the results of covariance analysis still showed that the mRNA expression levels of TWEAK,Fn14,IKKα and IKKβ in PBMC of the sarcopenia group were higher than those of the control group(all P<0.05).After adjusting for age and sex,partial correlation analysis showed that the expression of TWEAK mRNA was positively correlated with the expression of Fn14 mRNA(r=0.424,P<0.01),the expression of Fn14 mRNA was positively correlated with the mRNA expression lev-els of IKKα,IKKβ(r=0.264 and 0.284,respectively;both P<0.05),and the mRNA levels expression of IKKα,IKKβwere positively correlated with the expression of NF-κB p65 mRNA(r=0.724 and 0.660,respectively;both P<0.01).Conclusions TWEAK,Fn14,IKKα and IKKβ mRNA are highly expressed in PBMC of patients with senile sarcopenia in agricultural and pastoral areas of Xinjiang.The expression of TWEAK and Fn14 mRNA is correlated with the expression of IKKα and IKKβ mRNA.TWEAK and Fn14 may participate in the development and progression of senile sarcopenia by regulating IKK to activate NF-κB inflammatory pathway.