Effects of HYOU1 on autophagy and apoptosis of Schwann cells in high glucose environment and its mechanism
ZHOU Chenming
XUE Yan
MENG Li
ZHU Yan
XU Yannan
Abstract：Objective To explore the effects of hypoxia up-regulated 1(HYOU1)on autophagy and apoptosis of Schwann cells in the high glucose environment and to explore the related mechanism based on ROS/PI3K/AKT pathway.Methods RSC96 cells were cultured,HYOU1 recombinant expression plasmid was constructed,and the cells were ran-domly divided into the high glucose group,high glucose + control plasmid group,and high glucose +HYOU1 plasmid group.Cells in the three groups were all cultured with high glucose,and then cells in the high glucose + control plasmid group and high glucose +HYOU1 plasmid group were transfected with control plasmid and pEGFP-HYOU1 plasmid,re-spectively.Western blotting was used to detect autophagy-related proteins(LC3,ATG5,ATG12,Beclin1,p62),JAK-STAT pathway-related proteins(JAK1,JAK2,STAT1,STAT3,STAT5,STAT6),and ROS/PI3K/AKT pathway-related proteins(PI3K,AKT,mTOR,pPI3K,pAKT,pmTOR).Flow cytometry was used to detect the apoptosis rate.The apop-tosis-related genes Bax and Bcl-2 were detected by RT-PCR,and the ROS level in each group was detected by DCFH-DA staining.Results Compared with the high glucose group and high glucose+ control group,LC3,ATG5,ATG12 and Be-clin1 protein levels decreased and p62 protein level increased in the high glucose+HYOU1 group(all P<0.05).Compared with the high glucose group and high glucose+ control group,the apoptosis rate and Bax mRNA expression decreased,while Bcl-2 mRNA expression and the protein expression levels of JAK1,JAK2,STAT1,STAT3,STAT5 and STAT6 in-creased in the high glucose+HYOU1 group(all P<0.05).Compared with the high glucose group and high glucose+ control group,PI3K,AKT,mTOR,pPI3K,pAKT and pmTOR protein levels increased,and ROS level decreased in the high glu-cose+HYOU1 group(all P<0.05).Conclusion HYOU1 overexpression can down-regulate autophagy and apoptosis of Schwann cells in the high glucose environment,and the mechanism may be related to the regulation of ROS/PI3K/AKT pathway.
Keywords：HYOU1 geneSchwann cellsautophagyapoptosisROS/PI3K/AKT pathwaydiabetes mellitusdiabetic peripheral neuropathy
Publication Date：2023-11-15
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 6-10 )