Intervention of propofol on high glucose-induced injury in podocytes and its mechanism
WANG Ping
WANG Yang
GONG Xuemin
XUE Yan
Abstract：Objective To observe the intervention of propofol on high glucose-induced injury in podocytes and to ex-plore the related mechanisms based on nuclear factor κB(NF-κB)signaling pathway.Methods Human glomerular podocytes(HGPC)were cultured in vitro and the cells in the logarithmic growth phase were divided into the normal glu-cose group,high glucose group,propofol group,inhibitor group,propofol + inhibitor group,and propofol + activator group,respectively.Cells in the normal glucose group were treated with 5 mmol/L D-glucose.Cells in the other groups were treated with 30 mmol/L D-glucose,and cells in the propofol group were additionally given propofol(25,50,100 μmol/L),cells in the inhibitor group were given NF-κB signaling pathway inhibitor BAY 11-7082,cells in the propofol + inhibitor group were given propofol and BAY 11-7082,and cells in the propofol + activator group were given propofol and NF-κB signaling pathway activator Prostratin.After 24 h of intervention,CCK-8 was used to detect cell viability and we se-lected the optimal concentration of propofol.The cell morphology of each group was observed by inverted microscope.Tu-mor necrosis factor-α(TNF-α),interleukin-6(IL-6)and IL-1β in the supernatant of each group were determined by ELI-SA.The cell proliferation rate and cell migration number were measured by 5-acetylidene-2'deoxyuracil nucleoside meth-od and Transwell cell chamber method,respectively.Western blotting was used to determine epithelial-mesenchymal transformation(EMT)and NF-κB signaling pathway-related proteins in each group.Results The cross connections be-tween the foot processes were reduced,the cell bodies were reduced,and the cell spacing increased in the high glucose group.Podocyte morphological changes in the propofol group and the inhibitor group were less than those in the high glu-cose group;podocyte morphological changes in the propofol + inhibitor group were less than those in the propofol group,and podocyte morphological changes in the propofol + activator group were more than those in the propofol group.The ex-pression levels of TNF-α,IL-6,IL-1β,N-cadherin,Vimentin,p-NF-κB p65 protein and the number of migrating cells in the high glucose group were higher than those in the normal glucose group,while the cell proliferation rate and E-cadherin expression were lower than those in the normal glucose group(all P<0.05).The expression levels of TNF-α,IL-6,IL-1β,N-cadherin,Vimentin,p-NF-κB p65 protein and the number of migrating cells in the propofol group and the inhibitor group were lower than those in the high glucose group,and the cell proliferation rate and E-cadherin protein expression were higher than those in the high glucose group(all P<0.05).The expression levels of TNF-α,IL-6,IL-1β,N-cad-herin,Vimentin,p-NF-κB p65 protein and the number of migrating cells in the propofol + inhibitor group were lower than those in the propofol group,and the cell proliferation rate and E-cadherin protein expression were higher than those in the propofol group(all P<0.05).The expression levels of TNF-α,IL-6,IL-1β,N-cadherin,Vimentin,p-NF-κB p65 protein and the number of migrating cells in the propofol + activator group were higher than those in the propofol group,and the cell proliferation rate and E-cadherin protein expression were lower than those in the propofol group(all P<0.05).Conclusion Propofol could maintain the normal morphology of podocytes induced by high glucose,reduce inflammatory response,promote cell proliferation,inhibit cell migration and EMT,which may be related to the inhibition of NF-κB sig-naling transduction pathway.
Keywords：diabetic nephropathypropofolnuclear factor-κBinflammatory responsecell proliferationcell migrationepithelial-mesenchymal transformation
Publication Date：2023-11-15
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 1-5 )