Genetic polymorphism analysis of CYP2C19 in Han Chinese patients with coronary heart disease in Qinghai area
LI Yuxian
LIU Shiming
Abstract：Objective By analyzing the gene polymorphisms of CYP2C19,a key enzyme for clopidogrel metabo-lism,in Han Chinese patients with coronary heart disease(CHD)in Qinghai region,in order to provide a reference for the individualized and precise treatment of clinical antiplatelet drugs.Methods We selected 403 cases of Han Chinese pa-tients with CHD in Qinghai region,collected patients'venous blood,and analyzed CYP2C19 gene sequences by real-time fluorescence quantitative PCR.According to the PCR results,we determined CYP2C19 genotypes and metabolic pheno-types.Hardy-Weinberg equilibrium test was used to analyze the data's group representativeness,and we compared the metabolic phenotypes of CYP2C19 among different regions in the relevant literature.Results The allele frequencies of CYP2C19*1,CYP2C19*2,CYP2C19*3,and CYP2C19*17 in 403 Han Chinese CHD patients were 60.55%,30.77%,5.70%,and 2.98%,respectively.And the frequencies of CYP2C19*1/*1,*1/*2,*1/*3,*2/*2,* 2/*3,*3/*3,*1/*17,*2/*17,*3/*17 genotypes were 40.2%,30.8%,6.7%,12.2%,4.0%,0.2%,3.2%,2.5%,and 0.2%,re-spectively.The Hardy-Weinberg equilibrium test showed that the CYP2C19 genotype frequencies of Han Chinese CHD pa-tients in Qinghai were in accordance with the Hardy-weinberg equilibrium(P<0.05),with a good representation of the group.The frequencies of different metabolic phenotype in the patients were as follows:13 cases of ultra fast metabolizer(3.20%),162 cases of extensive metabolizer(40.2%),162 cases of intermediate metabolizer(40.2%)and 66 cases of poor metabolizer(16.4%).There were significant differences in the distribution of metabolic phenotypes between Han Chinese CHD patients in Qinghai area and Han Chinese CHD patients in Beijing,Gansu,Ningxia,Shanxi and Guangzhou areas(all P<0.05).Conclusions The genotypes of Han Chinese CHD patients in Qinghai region were dominated by CYP2C19*1/*1 and CYP2C19*1/*2.Compared with other regions in China,the risk of clopidogrel resistance was higher in CHD patients in Qinghai region.
Keywords：genetic polymorphismcoronary atherosclerotic cardiopathyclopidogrelCYP2C19Han nationalityQinghai region
Publication Date：2023-10-15
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 11-14 )