Expression of long non-coding RNA DANCR in colon cancer and its effect on invasion ability of LOVO cells
LI Tao
WANG Jiang
WANG Shu
ZHU Xuan
Abstract：Objective To observe expression of long non-coding RNA (lncRNA) differentiation antagonizing noncoding RNA (DANCR) in the colorectal cancer (CRC) cells and to explore its effect on the cell invasion. Methods The expression of DANCR in the CRC tissues and paired para-tumor tissues was determined by qRT-PCR. DANCR expression in the CRC patients with different clinicopathological features was measured. The qRT-PCR was applied to evaluate the expression of DANCR in the CRC cell line LOVO and normal epithelial cell line NCM460. LOVO cells were divided into the control group and DANCR-silenced group (or si-DANCR group) randomly, and were transfected with si-con and si-DANCR, respectively. The expression of DANCR was detected by qRT-PCR. We used the Transwell invasion assay to count the number of transmembrane cells and evaluate cell invasion. Western blotting was applied to detect the expression of invasion and metastasis-related factor matrix metalloproteinase 9 (MMP9). Results The expression of DANCR in the CRC tissues was higher than that in the para-tumor tissues. Additionally, the expression of DANCR in the LOVO cells was higher than that in the NCM460 cells (both P < 0.01). The expression of DANCR in the CRC patients with advanced clinical staging (stage Ⅲ and Ⅳ) , higher T staging (T3 and T4) , and distant metastasis was higher than that in CRC patients with early clinical staging (stage Ⅰ and Ⅱ) , lower T staging (T1 and T2) , and non-distant metastasis (P < 0.01). Compared with the control group, the expression of DANCR, the transmembrane cells, and the expression of MMP9 protein decreased in the si-DANCR group (all P < 0.01). Conclusion LncRNA DANCR is up-regulated in CRC tissues and cells, and DANCR works as an oncogene in the progression of CRC; DANCR promotes the metastatic ability of CRC cells, which is relatedto the up-regulation of MMP9.
Keywords：colon carcinomalong non-coding RNAdifferentiation antagonizing non-coding RNA (DANCR)tumor invasion
Publication Date：2019-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 29-32 )